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Plasmid Files

pcDNA™3.1/V5-His A

Mammalian vector for expressing C-terminally V5- and 6xHis-tagged proteins. For other reading frames, use pcDNA™3.1/V5‑His B or pcDNA™3.1/V5‑His C.

To see this sequence with restriction sites, features, and translations, please download
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pcDNA3.1 V5-His A.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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SgrDI (5500) SspI (5383) ScaI (5059) PvuI (4949) AhdI (4579) PciI (3686) BstZ17I (3307) BsmI (3255) PfoI (3110) RsrII (2849) BglII (12) MfeI (161) Bpu10I (180) NdeI (484) SnaBI (590) HindIII (902) Acc65I (908) KpnI (912) BamHI (920) EcoRI (943) EcoRV (955) NotI (970) PaeR7I - PspXI - XhoI (976) XbaI (982) PspOMI (988) ApaI (992) BstBI (995) AgeI (1045) PmeI (1075) BbsI (1286) DraIII (1600) SexAI * (1890) StuI (2122) AvrII (2123) TspMI - XmaI (2144) SmaI (2146) BsaBI * (2192) KasI (2332) NarI (2333) SfoI (2334) PluTI (2336) MscI (2415) PflFI - Tth111I (2451) BssHII (2730) pcDNA™3.1/V5-His A 5502 bp
SgrDI  (5500)
1 site
C G T C G A C G G C A G C T G C
SspI  (5383)
1 site
A A T A T T T T A T A A
ScaI  (5059)
1 site
A G T A C T T C A T G A
PvuI  (4949)
1 site
C G A T C G G C T A G C
AhdI  (4579)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (3686)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BstZ17I  (3307)
1 site
G T A T A C C A T A T G
BsmI  (3255)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
PfoI  (3110)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (2849)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BglII  (12)
1 site
A G A T C T T C T A G A
MfeI  (161)
1 site
C A A T T G G T T A A C
Bpu10I  (180)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.