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Plasmid Files

pcDNA™3.1/myc-His(-) C

Mammalian vector for expressing C-terminally Myc- and 6xHis-tagged proteins. For other reading frames, use pcDNA™3.1/myc‑His(–) A or pcDNA™3.1/myc‑His(–) B.

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pcDNA3.1 myc-His(-) C.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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SgrDI (5519) SspI (5402) ScaI (5078) PvuI (4968) AhdI (4598) PciI (3705) BstZ17I (3326) BsmI (3274) PfoI (3129) BstBI (3036) RsrII (2870) BglII (12) MfeI (161) Bpu10I (180) NruI (208) MluI (228) NdeI (484) NheI (895) BmtI (899) PspOMI (909) EcoO109I (910) ApaI (913) XbaI (915) PaeR7I - PspXI - XhoI (921) NotI (927) EcoRV (946) EcoRI (954) BamHI (977) Acc65I (989) KpnI (993) HindIII (995) AflII (1089) BbsI (1307) DraIII (1621) SexAI * (1911) BseRI (2140) StuI (2143) AvrII (2144) TspMI - XmaI (2165) SmaI (2167) BsaBI * (2213) KasI (2353) NarI (2354) SfoI (2355) PluTI (2357) MscI (2436) PflFI - Tth111I (2472) BssHII (2751) pcDNA™3.1/myc-His(-) C 5521 bp
SgrDI  (5519)
1 site
C G T C G A C G G C A G C T G C
SspI  (5402)
1 site
A A T A T T T T A T A A
ScaI  (5078)
1 site
A G T A C T T C A T G A
PvuI  (4968)
1 site
C G A T C G G C T A G C
AhdI  (4598)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (3705)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BstZ17I  (3326)
1 site
G T A T A C C A T A T G
BsmI  (3274)
1 site
G A A T G C N C T T A C G N