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Plasmid Files

pcDNA™4/TO/myc-His C

Vector for tetracycline-inducible expression of C-terminally Myc- and 6xHis-tagged proteins. For other reading frames, use pcDNA™4/TO/myc‑His A or pcDNA™4/TO/myc‑His B.

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pcDNA4 TO myc-His C.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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SspI (5028) ScaI (4704) PvuI (4594) FspI (4446) BsaI (4285) AhdI (4224) PciI (3331) BspQI - SapI (3215) BstZ17I (2952) BsmI (2900) FseI (2592) MfeI (161) Bpu10I (180) NruI (208) MluI (228) NdeI (484) SnaBI (590) BsmBI (893) BspEI (955) AflII (975) HindIII (978) Acc65I (984) KpnI (988) BamHI (996) EcoRI (1019) PstI (1028) EcoRV (1031) NotI (1046) PaeR7I - PspXI - XhoI (1052) BstEII (1057) BstBI (1067) AgeI (1108) StuI (2186) AvrII (2187) MscI (2319) BssHII - MauBI (2352) SgrAI (2430) BmgBI (2450) pcDNA™4/TO/myc-His C 5147 bp
SspI  (5028)
1 site
A A T A T T T T A T A A
ScaI  (4704)
1 site
A G T A C T T C A T G A
PvuI  (4594)
1 site
C G A T C G G C T A G C
FspI  (4446)
1 site
T G C G C A A C G C G T
BsaI  (4285)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (4224)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (3331)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (3215)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3215)
1 site
G C T C T T C N C G A G A A G N N