Resources
Plasmid Files

pcDNA™4/V5-His B

Mammalian vector for expressing C-terminally V5- and 6xHis-tagged proteins. For other reading frames, use pcDNA™4/V5‑His A or pcDNA™4/V5‑His C.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pcDNA4 V5-His B.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
Download Free Trial Get SnapGene Viewer


SgrDI (5094) SspI (4977) ScaI (4653) PvuI (4543) FspI (4395) AhdI (4173) PciI (3280) BspQI - SapI (3164) BstZ17I (2901) BsmI (2849) FseI (2541) BglII (12) MfeI (161) Bpu10I (180) NruI (208) NdeI (484) SnaBI (590) NheI (895) BmtI (899) AflII (908) HindIII (911) Acc65I (917) KpnI (921) BamHI (929) EcoRI (952) PstI (961) EcoRV (964) NotI (979) PaeR7I - PspXI - XhoI (985) XbaI (991) EcoO109I - PspOMI (997) ApaI (1001) SacII (1004) BstBI (1008) AgeI (1058) BbsI (1299) StuI (2135) AvrII (2136) MscI (2268) BssHII - MauBI (2301) SgrAI (2379) BmgBI (2399) pcDNA™4/V5-His B 5096 bp
SgrDI  (5094)
1 site
C G T C G A C G G C A G C T G C
SspI  (4977)
1 site
A A T A T T T T A T A A
ScaI  (4653)
1 site
A G T A C T T C A T G A
PvuI  (4543)
1 site
C G A T C G G C T A G C