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Plasmid Files

prHom-1

Mammalian vector encoding two FKBP homodimerizer domains, for creating cytoplasmic oligomers that can be dissociated with a drug. Also known as pC4EN-FM2E.

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prHom-1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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PvuII (5586) NaeI (5384) NgoMIV (5382) DraIII (5281) PfoI (4866) XmnI (4432) BsaI (3882) AhdI (3821) BspQI - SapI (2812) MluI (1) SnaBI (298) EcoRI (656) XbaI (682) SpeI (1336) BsiWI (1346) BamHI (1381) KflI - PpuMI (1410) HincII (1562) MfeI (1752) PspOMI (1927) ApaI (1931) Bsu36I (2031) MscI (2072) BstXI (2080) BglII (2101) PflMI (2307) AbsI - PaeR7I - PspXI - XhoI (2550) AvrII (2573) StuI (2578) SfiI (2627) prHom-1 5698 bp
PvuII  (5586)
1 site
C A G C T G G T C G A C
NaeI  (5384)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (5382)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
DraIII  (5281)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PfoI  (4866)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
XmnI  (4432)
1 site
G A A N N N N T T C C T T N N N N A A G
BsaI  (3882)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (3821)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BspQI  (2812)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2812)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
MluI  (1)
1 site
A C G C G T T G C G C A
SnaBI  (298)
1 site
T A C G T A A T G C A T
EcoRI  (656)
1 site
G A A T T C C T T A A G
XbaI  (682)
1 site
T C T A G A A G A T C T
SpeI  (1336)
1 site
A C T A G T T G A T C A
BsiWI  (1346)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
BamHI  (1381)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
KflI  (1410)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
PpuMI  (1410)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
HincII  (1562)
1 site
G T Y R A C C A R Y T G
MfeI  (1752)
1 site
C A A T T G G T T A A C
PspOMI  (1927)
1 site
G G G C C C C C C G G G
ApaI  (1931)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
Bsu36I  (2031)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
MscI  (2072)
1 site
T G G C C A A C C G G T
BstXI  (2080)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BglII  (2101)
1 site
A G A T C T T C T A G A
PflMI  (2307)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
AbsI  (2550)
1 site
C C T C G A G G G G A G C T C C
PaeR7I  (2550)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (2550)
1 site
V C T C G A G B B G A G C T C V
XhoI  (2550)
1 site
C T C G A G G A G C T C
AvrII  (2573)
1 site
C C T A G G G G A T C C
StuI  (2578)
1 site
A G G C C T T C C G G A
SfiI  (2627)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AmpR
3748 .. 4620  =  873 bp
290 amino acids  =  32.0 kDa
   Segment 2:  
   3748 .. 4551  =  804 bp
   267 amino acids  =  29.4 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3748 .. 4620  =  873 bp
290 amino acids  =  32.0 kDa
   Segment 1:  signal sequence  
   4552 .. 4620  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3748 .. 4620  =  873 bp
290 amino acids  =  32.0 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
2989 .. 3577  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2989 .. 3577  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
β-globin intron
1400 .. 1972  =  573 bp
intron from rabbit β-globin gene
β-globin intron
1400 .. 1972  =  573 bp
intron from rabbit β-globin gene
f1 ori
5057 .. 5512  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
5057 .. 5512  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
DmrD
688 .. 1008  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding protein FKBP12
dimerization can be reversed by ligand binding
DmrD
688 .. 1008  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding protein FKBP12
dimerization can be reversed by ligand binding
DmrD
1015 .. 1335  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding protein FKBP12
dimerization can be reversed by ligand binding
DmrD
1015 .. 1335  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding protein FKBP12
dimerization can be reversed by ligand binding
CMV enhancer
19 .. 322  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
19 .. 322  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
323 .. 526  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
323 .. 526  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 promoter
2557 .. 2752  =  196 bp
SV40 promoter
2557 .. 2752  =  196 bp
AmpR promoter
4621 .. 4725  =  105 bp
AmpR promoter
4621 .. 4725  =  105 bp
β-globin poly(A) signal
2169 .. 2224  =  56 bp
rabbit β-globin polyadenylation signal
β-globin poly(A) signal
2169 .. 2224  =  56 bp
rabbit β-globin polyadenylation signal
HSV TK 5'-UTR
616 .. 655  =  40 bp
5' untranslated region from the herpes simplex virus thymidine kinase gene
HSV TK 5'-UTR
616 .. 655  =  40 bp
5' untranslated region from the herpes simplex virus thymidine kinase gene
HA
1342 .. 1368  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
HA
1342 .. 1368  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
ATG
676 .. 678  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
676 .. 678  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
SV40 ori
2577 .. 2712  =  136 bp
SV40 origin of replication
SV40 ori
2577 .. 2712  =  136 bp
SV40 origin of replication
ORF:  676 .. 1371  =  696 bp
ORF:  231 amino acids  =  25.6 kDa
ORF:  3878 .. 4144  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  5528 .. 105  =  276 bp
ORF:  91 amino acids  =  9.5 kDa
ORF:  3748 .. 4620  =  873 bp
ORF:  290 amino acids  =  32.0 kDa
ORF:  405 .. 677  =  273 bp
ORF:  90 amino acids  =  9.7 kDa
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