Resources
Plasmid Files

pET-11b

Bacterial expression vector with a BamHI cloning site. For alternative reading frames, use pET‑11a or pET‑11c.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pET-11b Sequence and MappET-11b.dna
Map and Sequence File   
Sequence Author:  Novagen (EMD Millipore)
Download Free Trial Get SnapGene Viewer

 BspDI - ClaI (24) EcoRI (5674) AatII (5603) ZraI (5601) SspI (5485) ScaI (5161) PvuI (5051) PstI (4926) BsaI (4742) AhdI (4681) AlwNI (4204) PciI (3788) BspQI - SapI (3672) BstZ17I (3559) AccI (3558) BsaAI (3540) PflFI - Tth111I (3533) Bpu10I (2894) FspAI (2769) HindIII (29) BlpI (267) BamHI (319) T7 tag NheI (351) BmtI (355) NdeI (358) RBS XbaI (396) lac operator T7 promoter BglII (462) SgrAI (503) SphI (659) EcoNI (719) MluI (1184) BclI * (1198) BstEII (1365) PspOMI (1391) ApaI (1395) BssHII (1595) HpaI (1690) PshAI (2029) EagI (2252) NruI (2287) BfuAI - BspMI (2367) BsmI (2672) AvaI - BsoBI (2738) BmeT110I (2739) MscI * (2759) pET-11b 5676 bp
BspDI  (24)
1 site
A T C G A T T A G C T A
ClaI  (24)
1 site
A T C G A T T A G C T A
EcoRI  (5674)
1 site
G A A T T C C T T A A G
AatII  (5603)
1 site
G A C G T C C T G C A G
ZraI  (5601)
1 site
G A C G T C C T G C A G
SspI  (5485)
1 site
A A T A T T T T A T A A
ScaI  (5161)
1 site
A G T A C T T C A T G A
PvuI  (5051)
1 site
C G A T C G G C T A G C
PstI  (4926)
1 site
C T G C A G G A C G T C
BsaI  (4742)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (4681)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (4204)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (3788)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (3672)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3672)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
BstZ17I  (3559)
1 site
G T A T A C C A T A T G
AccI  (3558)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BsaAI  (3540)
1 site
Y A C G T R R T G C A Y
PflFI  (3533)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3533)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
Bpu10I  (2894)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
FspAI  (2769)
1 site
R T G C G C A Y Y A C G C G T R
HindIII  (29)
1 site
A A G C T T T T C G A A
BlpI  (267)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BamHI  (319)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
NheI  (351)
1 site
G C T A G C C G A T C G
BmtI  (355)
1 site
G C T A G C C G A T C G
NdeI  (358)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
XbaI  (396)
1 site
T C T A G A A G A T C T
BglII  (462)
1 site
A G A T C T T C T A G A
SgrAI  (503)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
SphI  (659)
1 site
G C A T G C C G T A C G
EcoNI  (719)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
MluI  (1184)
1 site
A C G C G T T G C G C A
BclI  (1198)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BstEII  (1365)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
PspOMI  (1391)
1 site
G G G C C C C C C G G G
ApaI  (1395)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BssHII  (1595)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
HpaI  (1690)
1 site
G T T A A C C A A T T G
PshAI  (2029)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
EagI  (2252)
1 site
C G G C C G G C C G G C
NruI  (2287)
1 site
T C G C G A A G C G C T
BfuAI  (2367)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (2367)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BsmI  (2672)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
AvaI  (2738)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (2738)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
BmeT110I  (2739)
1 site
C Y C G R G G R G C Y C
MscI  (2759)
1 site
T G G C C A A C C G G T
* Blocked by Dcm methylation.
lacI
834 .. 1916  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lacI
834 .. 1916  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
AmpR
4608 .. 5468  =  861 bp
286 amino acids  =  31.5 kDa
   Segment 2:  
   4608 .. 5399  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
4608 .. 5468  =  861 bp
286 amino acids  =  31.5 kDa
   Segment 1:  signal sequence  
   5400 .. 5468  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
4608 .. 5468  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
3849 .. 4437  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
3849 .. 4437  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
rop
3228 .. 3419  =  192 bp
63 amino acids  =  7.2 kDa
Product: Rop protein
rop
3228 .. 3419  =  192 bp
63 amino acids  =  7.2 kDa
Product: Rop protein
AmpR promoter
5469 .. 5573  =  105 bp
AmpR promoter
5469 .. 5573  =  105 bp
lacI promoter
756 .. 833  =  78 bp
lacI promoter
756 .. 833  =  78 bp
T7 terminator
213 .. 260  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
T7 terminator
213 .. 260  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
T7 tag
327 .. 359  =  33 bp
11 amino acids  =  1.1 kDa
Product: epitope tag from a T7 major capsid protein
T7 tag
327 .. 359  =  33 bp
11 amino acids  =  1.1 kDa
Product: epitope tag from a T7 major capsid protein
lac operator
404 .. 428  =  25 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
404 .. 428  =  25 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
T7 promoter
429 .. 447  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
429 .. 447  =  19 bp
promoter for bacteriophage T7 RNA polymerase
RBS
368 .. 373  =  6 bp
ribosome binding site
RBS
368 .. 373  =  6 bp
ribosome binding site
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter