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pET-41a(+)

Bacterial vector for expressing GST fusion proteins with an enterokinase site. For other reading frames, use pET‑41b(+) or pET‑41c(+).

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pET-41a(+).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Novagen (EMD Millipore)
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DraIII (5691) PsiI (5563) AsiSI - PvuI (4990) SmaI (4864) TspMI - XmaI (4862) NruI (4647) AcuI (4336) AlwNI (4204) BssS α I (3961) BstZ17I (3559) PflFI - Tth111I (3533) BlpI (80) AvrII (131) PaeR7I - PspXI - XhoI (174) EagI - NotI (182) HindIII (189) SalI (195) Eco53kI (204) SacI (206) PstI - SbfI (214) AscI (216) StuI (226) BsrGI (229) EcoRI (235) BamHI (241) EcoRV (251) NcoI (255) PshAI (265) AgeI (292) Acc65I (295) KpnI (299) BglII (302) MfeI (360) SacII (393) SpeI (424) SwaI (667) MscI (887) NdeI (1094) XbaI (1132) SgrAI (1243) SphI (1399) BstAPI (1607) MluI (1924) BstEII (2105) NmeAIII (2130) PspOMI (2131) ApaI (2135) HpaI (2430) PpuMI (2794) pET-41a(+) 5933 bp
DraIII  (5691)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PsiI  (5563)
1 site
T T A T A A A A T A T T
AsiSI  (4990)
1 site
G C G A T C G C C G C T A G C G
PvuI  (4990)
1 site
C G A T C G G C T A G C
SmaI  (4864)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (4862)
1 site
C C C G G G G G G C C C
XmaI  (4862)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
NruI  (4647)
1 site
T C G C G A A G C G C T
AcuI  (4336)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
AlwNI  (4204)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BssSαI  (3961)
1 site
C A C G A G G T G C T C
BstZ17I  (3559)
1 site
G T A T A C C A T A T G
PflFI  (3533)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3533)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BlpI  (80)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
AvrII  (131)
1 site
C C T A G G G G A T C C
PaeR7I  (174)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (174)
1 site
V C T C G A G B B G A G C T C V
XhoI  (174)
1 site
C T C G A G G A G C T C
EagI  (182)
1 site
C G G C C G G C C G G C
NotI  (182)
1 site
G C G G C C G C C G C C G G C G
HindIII  (189)
1 site
A A G C T T T T C G A A
SalI  (195)
1 site
G T C G A C C A G C T G
Eco53kI  (204)
1 site
G A G C T C C T C G A G
SacI  (206)
1 site
G A G C T C C T C G A G
PstI  (214)
1 site
C T G C A G G A C G T C
SbfI  (214)
1 site
C C T G C A G G G G A C G T C C
AscI  (216)
1 site
G G C G C G C C C C G C G C G G
StuI  (226)
1 site
A G G C C T T C C G G A
BsrGI  (229)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
EcoRI  (235)
1 site
G A A T T C C T T A A G
BamHI  (241)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
EcoRV  (251)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
NcoI  (255)
1 site
C C A T G G G G T A C C
PshAI  (265)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
AgeI  (292)
1 site
A C C G G T