Resources
Plasmid Files

pET-41b(+)

Bacterial vector for expressing GST fusion proteins with an enterokinase site. For other reading frames, use pET‑41a(+) or pET‑41c(+).

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pET-41b(+).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Novagen (EMD Millipore)
Download Free Trial Get SnapGene Viewer

 BlpI (80) DraIII (5690) PsiI (5562) AsiSI - PvuI (4989) SmaI (4863) TspMI - XmaI (4861) NruI (4646) AcuI (4335) AlwNI (4203) BssS α I (3960) BstZ17I (3558) PflFI - Tth111I (3532) AvrII (131) 8xHis PaeR7I - PspXI - XhoI (174) EagI - NotI (182) HindIII (189) SalI (195) Eco53kI (204) SacI (206) PstI - SbfI (214) AscI (216) StuI (226) BsrGI (229) EcoRI (235) BamHI (241) EcoRV (251) NcoI (254) PshAI (264) AgeI (291) Acc65I (294) KpnI (298) BglII (301) S-Tag MfeI (359) SacII (392) 6xHis SpeI (423) SwaI (666) MscI (886) NdeI (1093) RBS XbaI (1131) T7 promoter SgrAI (1242) SphI (1398) BstAPI (1606) MluI (1923) BstEII (2104) NmeAIII (2129) PspOMI (2130) ApaI (2134) HpaI (2429) PpuMI (2793) pET-41b(+) 5932 bp
BlpI  (80)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
DraIII  (5690)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PsiI  (5562)
1 site
T T A T A A A A T A T T
AsiSI  (4989)
1 site
G C G A T C G C C G C T A G C G
PvuI  (4989)
1 site
C G A T C G G C T A G C
SmaI  (4863)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (4861)
1 site
C C C G G G G G G C C C
XmaI  (4861)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
NruI  (4646)
1 site
T C G C G A A G C G C T
AcuI  (4335)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
AlwNI  (4203)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BssSαI  (3960)
1 site
C A C G A G G T G C T C
BstZ17I  (3558)
1 site
G T A T A C C A T A T G
PflFI  (3532)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3532)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
AvrII  (131)
1 site
C C T A G G G G A T C C
PaeR7I  (174)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (174)
1 site
V C T C G A G B B G A G C T C V
XhoI  (174)
1 site
C T C G A G G A G C T C
EagI  (182)
1 site
C G G C C G G C C G G C
NotI  (182)
1 site
G C G G C C G C C G C C G G C G
HindIII  (189)
1 site
A A G C T T T T C G A A
SalI  (195)
1 site
G T C G A C C A G C T G
Eco53kI  (204)
1 site
G A G C T C C T C G A G
SacI  (206)
1 site
G A G C T C C T C G A G
PstI  (214)
1 site
C T G C A G G A C G T C
SbfI  (214)
1 site
C C T G C A G G G G A C G T C C
AscI  (216)
1 site
G G C G C G C C C C G C G C G G
StuI  (226)
1 site
A G G C C T T C C G G A
BsrGI  (229)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
EcoRI  (235)
1 site
G A A T T C C T T A A G
BamHI  (241)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
EcoRV  (251)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
NcoI  (254)
1 site
C C A T G G G G T A C C
PshAI  (264)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
AgeI  (291)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
Acc65I  (294)
1 site
G G T A C C C C A T G G
KpnI  (298)
1 site
G G T A C C C C A T G G
BglII  (301)
1 site
A G A T C T T C T A G A
MfeI  (359)
1 site
C A A T T G G T T A A C
SacII  (392)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
SpeI  (423)
1 site
A C T A G T T G A T C A
SwaI  (666)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
MscI  (886)
1 site
T G G C C A A C C G G T
NdeI  (1093)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
XbaI  (1131)
1 site
T C T A G A A G A T C T
SgrAI  (1242)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
SphI  (1398)
1 site
G C A T G C C G T A C G
BstAPI  (1606)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
MluI  (1923)
1 site
A C G C G T T G C G C A
BstEII  (2104)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
NmeAIII  (2129)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PspOMI  (2130)
1 site
G G G C C C C C C G G G
ApaI  (2134)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
HpaI  (2429)
1 site
G T T A A C C A A T T G
PpuMI  (2793)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
lacI
1573 .. 2655  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lacI
1573 .. 2655  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
KanR
4558 .. 5373  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
KanR
4558 .. 5373  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
GST
441 .. 1094  =  654 bp
218 amino acids  =  25.5 kDa
Product: glutathione S-transferase from
Schistosoma japonicum
GST
441 .. 1094  =  654 bp
218 amino acids  =  25.5 kDa
Product: glutathione S-transferase from
Schistosoma japonicum
ori
3848 .. 4436  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
3848 .. 4436  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
5466 .. 5921  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
5466 .. 5921  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
rop
3227 .. 3418  =  192 bp
63 amino acids  =  7.2 kDa
Product: Rop protein
rop
3227 .. 3418  =  192 bp
63 amino acids  =  7.2 kDa
Product: Rop protein
MCS
174 .. 264  =  91 bp
multiple cloning site
MCS
174 .. 264  =  91 bp
multiple cloning site
lacI promoter
1495 .. 1572  =  78 bp
lacI promoter
1495 .. 1572  =  78 bp
T7 terminator
26 .. 73  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
T7 terminator
26 .. 73  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
S-Tag
309 .. 353  =  45 bp
15 amino acids  =  1.7 kDa
Product: affinity and epitope tag derived from
pancreatic ribonuclease A
S-Tag
309 .. 353  =  45 bp
15 amino acids  =  1.7 kDa
Product: affinity and epitope tag derived from
pancreatic ribonuclease A
lac operator
1139 .. 1163  =  25 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
1139 .. 1163  =  25 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
8xHis
150 .. 173  =  24 bp
8 amino acids  =  1.1 kDa
Product: 8xHis affinity tag
8xHis
150 .. 173  =  24 bp
8 amino acids  =  1.1 kDa
Product: 8xHis affinity tag
T7 promoter
1164 .. 1182  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
1164 .. 1182  =  19 bp
promoter for bacteriophage T7 RNA polymerase
thrombin site
369 .. 386  =  18 bp
6 amino acids  =  627.8 Da
Product: thrombin recognition and cleavage site
thrombin site
369 .. 386  =  18 bp
6 amino acids  =  627.8 Da
Product: thrombin recognition and cleavage site
6xHis
396 .. 413  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
396 .. 413  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
RBS
1103 .. 1108  =  6 bp
ribosome binding site
RBS
1103 .. 1108  =  6 bp
ribosome binding site
enterokinase site
264 .. 278  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
enterokinase site
264 .. 278  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2017 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter