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Plasmid Files

pET-43.1 Ek/LIC

Bacterial vector for ligation-independent cloning (LIC) to express NusA-tagged proteins with an enterokinase site.

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pET-43.1 Ek_LIC.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Novagen (EMD Millipore)
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DraIII (7053) PsiI (6925) AhdI (6641) BglI (6523) FspI (6418) ScaI (6160) AlwNI (5447) PciI (5031) BspQI - SapI (4915) PflFI - Tth111I (4776) Bpu10I (4137) PpuMI (4037) HpaI (3673) AvrII (483) PacI (503) PaeR7I - XhoI (530) PmlI (576) EagI - NotI (592) HindIII (599) Acc65I (620) KpnI (624) SalI - SgrDI (626) PstI - SbfI (639) AscI (641) BsrGI (648) EcoRI (654) BamHI (660) Eco53kI (670) SacI (672) BseRI (699) PshAI (701) TspMI - XmaI (739) SmaI (741) SacII (817) SpeI (848) BglII (963) BsmI (1007) MscI (1095) StuI (1148) BfuAI - BspMI (1297) NcoI (1423) NdeI (2337) XbaI (2375) SphI (2642) EcoNI (2702) BstEII (3348) PspOMI (3374) ApaI (3378) pET-43.1 Ek/LIC 7295 bp
DraIII  (7053)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PsiI  (6925)
1 site
T T A T A A A A T A T T
AhdI  (6641)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BglI  (6523)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
FspI  (6418)
1 site
T G C G C A A C G C G T
ScaI  (6160)
1 site
A G T A C T T C A T G A
AlwNI  (5447)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (5031)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (4915)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (4915)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PflFI  (4776)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4776)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
Bpu10I  (4137)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
PpuMI  (4037)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
HpaI  (3673)
1 site
G T T A A C C A A T T G
AvrII  (483)
1 site
C C T A G G G G A T C C
PacI  (503)
1 site
T T A A T T A A A A T T A A T T