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pET-43.1 Ek/LIC (linearized)

Linearized bacterial vector for ligation-independent cloning (LIC) to express NusA-tagged proteins with an enterokinase site.

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pET-43.1 Ek_LIC (linearized).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Novagen (EMD Millipore)
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DraIII (6354) PsiI (6226) AhdI (5942) BglI (5824) FspI (5719) ScaI (5461) AlwNI (4748) PciI (4332) BspQI - SapI (4216) PflFI - Tth111I (4077) Bpu10I (3438) PpuMI (3338) HpaI (2974) ApaI (2679) AvrII (7079) PacI (7099) PaeR7I - XhoI (7126) PmlI (7172) EagI - NotI (7188) HindIII (7195) Acc65I (7216) KpnI (7220) SalI - SgrDI (7222) PstI - SbfI (7235) AscI (7237) BsrGI (7244) EcoRI (7250) BamHI (7256) Eco53kI (7266) SacI (7268) End (7279) Start (0) TspMI - XmaI (40) SmaI (42) SacII (118) SpeI (149) BglII (264) BsmI (308) MscI (396) StuI (449) BfuAI - BspMI (598) NcoI (724) NdeI (1638) XbaI (1676) SphI (1943) EcoNI (2003) BstEII (2649) PspOMI (2675) pET-43.1 Ek/LIC 7279 bp
DraIII  (6354)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PsiI  (6226)
1 site
T T A T A A A A T A T