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Plasmid Files

pET-43.1c(+)

Bacterial vector for expressing NusA fusion proteins. For other reading frames, use pET‑43.1a(+) or pET‑43.1b(+).

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pET-43.1c(+).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Novagen (EMD Millipore)
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DraIII (7031) PsiI (6903) AhdI (6619) BglI (6501) FspI (6396) ScaI (6138) AlwNI (5425) PciI (5009) BspQI - SapI (4893) PflFI - Tth111I (4754) Bpu10I (4115) PpuMI (4015) HpaI (3651) AvrII (483) PacI (503) PaeR7I - XhoI (530) PmlI (576) EagI - NotI (592) HindIII (599) Acc65I (620) KpnI (624) SalI - SgrDI (626) PstI - SbfI (639) AscI (641) BsrGI (648) EcoRI (654) BamHI (660) Eco53kI (670) SacI (672) PshAI (679) TspMI - XmaI (717) SmaI (719) SacII (795) SpeI (826) BglII (941) BsmI (985) MscI (1073) StuI (1126) BfuAI - BspMI (1275) NcoI (1401) NdeI (2315) XbaI (2353) SphI (2620) EcoNI (2680) BstEII (3326) PspOMI (3352) ApaI (3356) pET-43.1c(+) 7273 bp
DraIII  (7031)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PsiI  (6903)
1 site
T T A T A A A A T A T T
AhdI  (6619)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BglI  (6501)
1 site
G C C N N N N N G G C C G G N