Resources
Plasmid Files

pETcoco™-2

Bacterial vector with an ampicillin resistance marker that allows single-copy replication, on-demand amplification, and tightly regulated expression.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pETcoco-2 Sequence and MappETcoco-2.dna
Map and Sequence File   
Sequence Author:  Novagen (EMD Millipore)
Download Free Trial Get SnapGene Viewer

 T7 promoter PciI (10,151) NcoI (9856) BsaI (9479) SpeI (9391) BseRI (8369) SnaBI (8300) PflFI - Tth111I (7940) KpnI (7880) Acc65I (7876) SbfI (6694) BsrGI (6449) FspAI (6421) lac operator RBS SphI (74) NsiI (76) NheI (96) BmtI (100) SrfI (181) AsiSI (188) ZraI (200) AatII (202) AscI (207) PmlI (215) BsiWI (219) BstBI (228) HindIII (231) NotI (238) PacI (288) Bsu36I (293) SwaI (302) AvrII (309) PspXI (320) 6xHis BamHI (1974) EcoRI (2050) SfiI (2434) AarI (2679) ScaI (3999) FseI (5567) SacII (5571) StuI (5843) pETcoco™-2 12,417 bp
PciI  (10,151)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
NcoI  (9856)
1 site
C C A T G G G G T A C C
BsaI  (9479)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
SpeI  (9391)
1 site
A C T A G T T G A T C A
BseRI  (8369)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
SnaBI  (8300)
1 site
T A C G T A A T G C A T
PflFI  (7940)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (7940)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
KpnI  (7880)
1 site
G G T A C C C C A T G G
Acc65I  (7876)
1 site
G G T A C C C C A T G G
SbfI  (6694)
1 site
C C T G C A G G G G A C G T C C
BsrGI  (6449)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
FspAI  (6421)
1 site
R T G C G C A Y Y A C G C G T R
SphI  (74)
1 site
G C A T G C C G T A C G
NsiI  (76)
1 site
A T G C A T T A C G T A
NheI  (96)
1 site
G C T A G C C G A T C G
BmtI  (100)
1 site
G C T A G C C G A T C G
SrfI  (181)
1 site
G C C C G G G C C G G G C C C G
AsiSI  (188)
1 site
G C G A T C G C C G C T A G C G
ZraI  (200)
1 site
G A C G T C C T G C A G
AatII  (202)
1 site
G A C G T C C T G C A G
AscI  (207)
1 site
G G C G C G C C C C G C G C G G
PmlI  (215)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
BsiWI  (219)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
BstBI  (228)
1 site
T T C G A A A A G C T T
HindIII  (231)
1 site
A A G C T T T T C G A A
NotI  (238)
1 site
G C G G C C G C C G C C G G C G
PacI  (288)
1 site
T T A A T T A A A A T T A A T T
Bsu36I  (293)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
SwaI  (302)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
AvrII  (309)
1 site
C C T A G G G G A T C C
PspXI  (320)
1 site
V C T C G A G B B G A G C T C V
BamHI  (1974)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
EcoRI  (2050)
1 site
G A A T T C C T T A A G
SfiI  (2434)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AarI  (2679)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI
recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its
electrophoretic mobility.
ScaI  (3999)
1 site
A G T A C T T C A T G A
FseI  (5567)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
SacII  (5571)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
StuI  (5843)
1 site
A G G C C T T C C G G A
sopA
7400 .. 8575  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F
plasmid
sopA
7400 .. 8575  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F
plasmid
trfA
2080 .. 3228  =  1149 bp
382 amino acids  =  43.8 kDa
Product: trans-acting replication protein that binds
to and activates oriV
trfA
2080 .. 3228  =  1149 bp
382 amino acids  =  43.8 kDa
Product: trans-acting replication protein that binds
to and activates oriV
lacI
10,928 .. 12,010  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lacI
10,928 .. 12,010  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
araC
831 .. 1709  =  879 bp
292 amino acids  =  33.4 kDa
Product: L-arabinose regulatory protein
araC
831 .. 1709  =  879 bp
292 amino acids  =  33.4 kDa
Product: L-arabinose regulatory protein
AmpR
3446 .. 4306  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   3446 .. 4234  =  789 bp
   262 amino acids  =  28.8 kDa
AmpR
3446 .. 4306  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   4235 .. 4306  =  72 bp
   24 amino acids  =  2.8 kDa
AmpR
3446 .. 4306  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
repE
6066 .. 6821  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the
bacterial F plasmid
repE
6066 .. 6821  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the
bacterial F plasmid
oriV
5042 .. 5656  =  615 bp
origin of replication for the bacterial F plasmid
oriV
5042 .. 5656  =  615 bp
origin of replication for the bacterial F plasmid
sopC
9619 .. 10,092  =  474 bp
centromere-like partitioning region of the bacterial F
plasmid
sopC
9619 .. 10,092  =  474 bp
centromere-like partitioning region of the bacterial F
plasmid
cos
10,352 .. 10,750  =  399 bp
lambda cos site; allows packaging into phage
lambda particles
cos
10,352 .. 10,750  =  399 bp
lambda cos site; allows packaging into phage
lambda particles
incC
6824 .. 7074  =  251 bp
incompatibility region of the bacterial F plasmid
incC
6824 .. 7074  =  251 bp
incompatibility region of the bacterial F plasmid
MCS
70 .. 314  =  245 bp
multiple cloning site
MCS
70 .. 314  =  245 bp
multiple cloning site
ori2
5756 .. 5975  =  220 bp
secondary origin of replication for the bacterial F
plasmid; also known as oriS
ori2
5756 .. 5975  =  220 bp
secondary origin of replication for the bacterial F
plasmid; also known as oriS
araBAD promoter
1855 .. 2020  =  166 bp
promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction
araBAD promoter
1855 .. 2020  =  166 bp
promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction
AmpR promoter
4307 .. 4398  =  92 bp
AmpR promoter
4307 .. 4398  =  92 bp
lacI promoter
12,011 .. 12,088  =  78 bp
lacI promoter
12,011 .. 12,088  =  78 bp
T7 terminator
410 .. 457  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
T7 terminator
410 .. 457  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
loxP
10,768 .. 10,801  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
loxP
10,768 .. 10,801  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
lac operator
3 .. 27  =  25 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
3 .. 27  =  25 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
T7 promoter
12,401 .. 2  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
12,401 .. 2  =  19 bp
promoter for bacteriophage T7 RNA polymerase
6xHis
326 .. 343  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
326 .. 343  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
RBS
58 .. 63  =  6 bp
ribosome binding site
RBS
58 .. 63  =  6 bp
ribosome binding site
sopB
8575 .. 9546  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F
plasmid
sopB
8575 .. 9546  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F
plasmid
S-Tag
102 .. 146  =  45 bp
15 amino acids  =  1.7 kDa
Product: affinity and epitope tag derived from
pancreatic ribonuclease A
S-Tag
102 .. 146  =  45 bp
15 amino acids  =  1.7 kDa
Product: affinity and epitope tag derived from
pancreatic ribonuclease A
HSV tag
249 .. 281  =  33 bp
11 amino acids  =  1.2 kDa
Product: HSV (herpes simplex virus) epitope tag
HSV tag
249 .. 281  =  33 bp
11 amino acids  =  1.2 kDa
Product: HSV (herpes simplex virus) epitope tag
6xHis
75 .. 92  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
75 .. 92  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
enterokinase site
162 .. 176  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
enterokinase site
162 .. 176  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
ATG
72 .. 74  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
72 .. 74  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter