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Plasmid Files

pBINPLUS

Improved binary Agrobacterium vector for plant transformation.

 
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 EcoNI (10,768) PacI (9595) EcoRI (9585) SacI (9583) Eco53kI (9581) KpnI (9577) Acc65I (9573) SmaI (9571) TspMI - XmaI (9569) BamHI (9564) XbaI (9558) SalI (9552) SbfI (9550) HindIII (9534) AscI (9527) lac operator XmnI (9230) BspDI - ClaI (9108) MauBI (8817) AflII (8622) ApaI (8474) PspOMI (8470) BstBI (8219) BmtI (7290) NheI (7286) Bsu36I (7271) AhdI (6401) BbvCI (6343) KflI (6253) NotI (92) PasI (645) PshAI (1412) MluI (1649) BstEII (1752) ApaLI (1852) BstXI (2686) SfiI (4318) PflMI * (4647) NdeI (4876) AleI (5002) MfeI (5272) FspAI (5658) NruI (6155) pBINPLUS 12,396 bp
EcoNI  (10,768)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
PacI  (9595)
1 site
T T A A T T A A A A T T A A T T
EcoRI  (9585)
1 site
G A A T T C C T T A A G
SacI  (9583)
1 site
G A G C T C C T C G A G
Eco53kI  (9581)
1 site
G A G C T C C T C G A G
KpnI  (9577)
1 site
G G T A C C C C A T G G
Acc65I  (9573)
1 site
G G T A C C C C A T G G
SmaI  (9571)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (9569)
1 site
C C C G G G G G G C C C
XmaI  (9569)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
BamHI  (9564)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
XbaI  (9558)
1 site
T C T A G A A G A T C T
SalI  (9552)
1 site
G T C G A C C A G C T G
SbfI  (9550)
1 site
C C T G C A G G G G A C G T C C
HindIII  (9534)
1 site
A A G C T T T T C G A A
AscI  (9527)
1 site
G G C G C G C C C C G C G C G G
XmnI  (9230)
1 site
G A A N N N N T T C C T T N N N N A A G
BspDI  (9108)
1 site
A T C G A T T A G C T A
ClaI  (9108)
1 site
A T C G A T T A G C T A
MauBI  (8817)
1 site
C G C G C G C G G C G C G C G C
AflII  (8622)
1 site
C T T A A G G A A T T C

The sticky ends produced by AflII are hard to ligate.
ApaI  (8474)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (8470)
1 site
G G G C C C C C C G G G
BstBI  (8219)
1 site
T T C G A A A A G C T T
BmtI  (7290)
1 site
G C T A G C C G A T C G
NheI  (7286)
1 site
G C T A G C C G A T C G
Bsu36I  (7271)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
AhdI  (6401)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BbvCI  (6343)
1 site
C C T C A G C G G A G T C G
KflI  (6253)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
NotI  (92)
1 site
G C G G C C G C C G C C G G C G
PasI  (645)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
PshAI  (1412)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
MluI  (1649)
1 site
A C G C G T T G C G C A
BstEII  (1752)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
ApaLI  (1852)
1 site
G T G C A C C A C G T G
BstXI  (2686)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
SfiI  (4318)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
PflMI  (4647)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
NdeI  (4876)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
AleI  (5002)
1 site
C A C N N N N G T G G T G N N N N C A C
MfeI  (5272)
1 site
C A A T T G G T T A A C
FspAI  (5658)
1 site
R T G C G C A Y Y A C G C G T R
NruI  (6155)
1 site
T C G C G A A G C G C T
trfA
3964 .. 5112  =  1149 bp
382 amino acids  =  43.8 kDa
Product: trans-acting replication protein that binds
to and activates oriV
trfA
3964 .. 5112  =  1149 bp
382 amino acids  =  43.8 kDa
Product: trans-acting replication protein that binds
to and activates oriV
KanR
2871 .. 3665  =  795 bp
264 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
KanR
2871 .. 3665  =  795 bp
264 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
NeoR/KanR
7409 .. 8203  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin®)
NeoR/KanR
7409 .. 8203  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin®)
oriV
12,379 .. 694  =  712 bp
incP origin of replication
oriV
12,379 .. 694  =  712 bp
incP origin of replication
TetR
10,408 .. 11,058  =  651 bp
216 amino acids  =  23.3 kDa
Product: tetracycline resistance regulatory protein
TetR
10,408 .. 11,058  =  651 bp
216 amino acids  =  23.3 kDa
Product: tetracycline resistance regulatory protein
traJ
11,881 .. 12,252  =  372 bp
123 amino acids  =  13.5 kDa
Product: oriT-recognizing protein
traJ
11,881 .. 12,252  =  372 bp
123 amino acids  =  13.5 kDa
Product: oriT-recognizing protein
pBR322 ori (truncated)
1758 .. 2110  =  353 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication (truncated)
pBR322 ori (truncated)
1758 .. 2110  =  353 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication (truncated)
lacZα
9504 .. 9854  =  351 bp
116 amino acids  =  13.1 kDa
Product: LacZα fragment of β-galactosidase
lacZα
9504 .. 9854  =  351 bp
116 amino acids  =  13.1 kDa
Product: LacZα fragment of β-galactosidase
NOS terminator
8593 .. 8845  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS terminator
8593 .. 8845  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS promoter
7173 .. 7352  =  180 bp
nopaline synthase promoter
NOS promoter
7173 .. 7352  =  180 bp
nopaline synthase promoter
oriT
11,739 .. 11,848  =  110 bp
incP origin of transfer
oriT
11,739 .. 11,848  =  110 bp
incP origin of transfer
lac promoter
9430 .. 9460  =  31 bp
   Segment 1:  -35  
   9430 .. 9435  =  6 bp
promoter for the E. coli lac operon
lac promoter
9430 .. 9460  =  31 bp
   Segment 2:  
   9436 .. 9453  =  18 bp
promoter for the E. coli lac operon
lac promoter
9430 .. 9460  =  31 bp
   Segment 3:  -10  
   9454 .. 9460  =  7 bp
promoter for the E. coli lac operon
lac promoter
9430 .. 9460  =  31 bp
3 segments
promoter for the E. coli lac operon
LB T-DNA repeat
6708 .. 6732  =  25 bp
left border repeat from nopaline C58 T-DNA
LB T-DNA repeat
6708 .. 6732  =  25 bp
left border repeat from nopaline C58 T-DNA
RB T-DNA repeat
9919 .. 9943  =  25 bp
right border repeat from nopaline C58 T-DNA
RB T-DNA repeat
9919 .. 9943  =  25 bp
right border repeat from nopaline C58 T-DNA
lac operator
9468 .. 9484  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
9468 .. 9484  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
MCS
9526 .. 9598  =  73 bp
multiple cloning site
MCS
9526 .. 9598  =  73 bp
multiple cloning site
M13 rev
9492 .. 9508  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
9492 .. 9508  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
9605 .. 9621  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
9605 .. 9621  =  17 bp
common sequencing primer, one of multiple similar
variants
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