Resources
Plasmid Files

pCAMBIA1304

Agrobacterium binary vector for plant transformation, with hygromycin- and kanamycin-resistance and GFP-GUS genes.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pCAMBIA1304 Sequence and MappCAMBIA1304.dna
Map and Sequence File   
Sequence Author:  Cambia
Download Free Trial Get SnapGene Viewer

 NcoI (0) HindIII (11,600) PstI - SbfI (11,592) SalI (11,582) XbaI (11,576) BamHI (11,570) KpnI (11,565) Acc65I (11,561) BanII - SacI (11,559) Eco53kI (11,557) EcoRI (11,549) lac operator lac promoter BstXI (11,306) AatII (10,465) ZraI (10,463) AsiSI (10,126) RsrII (10,077) PspXI (9425) SacII (8907) PsiI (8779) Bpu10I (8634) BlpI (8303) PluTI (6771) SfoI (6769) NarI (6768) KasI (6767) MreI - SgrAI (6764) EcoNI (6295) BglII (7) SpeI (14) Bsu36I (279) SnaBI (1115) 6xHis BstEII (2573) MauBI (2819) RB T-DNA repeat PasI (4312) AclI (4906) BsiWI (5866) pCAMBIA1304 12,362 bp
NcoI  (0)
1 site
C C A T G G G G T A C C
HindIII  (11,600)
1 site
A A G C T T T T C G A A
PstI  (11,592)
1 site
C T G C A G G A C G T C
SbfI  (11,592)
1 site
C C T G C A G G G G A C G T C C
SalI  (11,582)
1 site
G T C G A C C A G C T G
XbaI  (11,576)
1 site
T C T A G A A G A T C T
BamHI  (11,570)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
KpnI  (11,565)
1 site
G G T A C C C C A T G G
Acc65I  (11,561)
1 site
G G T A C C C C A T G G
BanII  (11,559)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
SacI  (11,559)
1 site
G A G C T C C T C G A G
Eco53kI  (11,557)
1 site
G A G C T C C T C G A G
EcoRI  (11,549)
1 site
G A A T T C C T T A A G
BstXI  (11,306)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
AatII  (10,465)
1 site
G A C G T C C T G C A G
ZraI  (10,463)
1 site
G A C G T C C T G C A G
AsiSI  (10,126)
1 site
G C G A T C G C C G C T A G C G
RsrII  (10,077)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PspXI  (9425)
1 site
V C T C G A G B B G A G C T C V
SacII  (8907)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
PsiI  (8779)
1 site
T T A T A A A A T A T T
Bpu10I  (8634)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BlpI  (8303)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
PluTI  (6771)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (6769)
1 site
G G C G C C C C G C G G
NarI  (6768)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (6767)
1 site
G G C G C C C C G C G G
MreI  (6764)
1 site
C G C C G G C G G C G G C C G C
SgrAI  (6764)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
EcoNI  (6295)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BglII  (7)
1 site
A G A T C T T C T A G A
SpeI  (14)
1 site
A C T A G T T G A T C A
Bsu36I  (279)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
SnaBI  (1115)
1 site
T A C G T A A T G C A T
BstEII  (2573)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
MauBI  (2819)
1 site
C G C G C G C G G C G C G C G C
PasI  (4312)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
AclI  (4906)
1 site
A A C G T T T T G C A A
BsiWI  (5866)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
GUS
734 .. 2539  =  1806 bp
602 amino acids  =  68.3 kDa
Product: β-glucuronidase
GUS
734 .. 2539  =  1806 bp
602 amino acids  =  68.3 kDa
Product: β-glucuronidase
pVS1 RepA
5251 .. 6324  =  1074 bp
357 amino acids  =  39.9 kDa
Product: replication protein from Pseudomonas
plasmid pVS1
pVS1 RepA
5251 .. 6324  =  1074 bp
357 amino acids  =  39.9 kDa
Product: replication protein from Pseudomonas
plasmid pVS1
HygR
9464 .. 10,489  =  1026 bp
341 amino acids  =  38.0 kDa
Product: hygromycin B phosphotransferase
confers resistance to hygromycin
HygR
9464 .. 10,489  =  1026 bp
341 amino acids  =  38.0 kDa
Product: hygromycin B phosphotransferase
confers resistance to hygromycin
KanR
7929 .. 8723  =  795 bp
264 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
KanR
7929 .. 8723  =  795 bp
264 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
mgfp5
17 .. 727  =  711 bp
237 amino acids  =  26.7 kDa
Product: GFP with folding enhancement mutations
suitable for expression in plants due to removal of a
cryptic intron
mgfp5
17 .. 727  =  711 bp
237 amino acids  =  26.7 kDa
Product: GFP with folding enhancement mutations
suitable for expression in plants due to removal of a
cryptic intron
CaMV 35S promoter (enhanced)
10,557 .. 11,234  =  678 bp
cauliflower mosaic virus 35S promoter with a
duplicated enhancer region
CaMV 35S promoter (enhanced)
10,557 .. 11,234  =  678 bp
cauliflower mosaic virus 35S promoter with a
duplicated enhancer region
pVS1 StaA
4193 .. 4822  =  630 bp
209 amino acids  =  22.1 kDa
Product: stability protein from Pseudomonas
plasmid pVS1
pVS1 StaA
4193 .. 4822  =  630 bp
209 amino acids  =  22.1 kDa
Product: stability protein from Pseudomonas
plasmid pVS1
ori
7254 .. 7842  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
7254 .. 7842  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
CaMV 35S promoter
12,002 .. 12,347  =  346 bp
strong constitutive promoter from cauliflower
mosaic virus
CaMV 35S promoter
12,002 .. 12,347  =  346 bp
strong constitutive promoter from cauliflower
mosaic virus
NOS terminator
2595 .. 2847  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS terminator
2595 .. 2847  =  253 bp
nopaline synthase terminator and poly(A) signal
lacZα
11,535 .. 11,768  =  234 bp
77 amino acids  =  8.6 kDa
Product: LacZα fragment of β-galactosidase
lacZα
11,535 .. 11,768  =  234 bp
77 amino acids  =  8.6 kDa
Product: LacZα fragment of β-galactosidase
pVS1 oriV
6390 .. 6584  =  195 bp
origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)
pVS1 oriV
6390 .. 6584  =  195 bp
origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)
CaMV poly(A) signal
9250 .. 9424  =  175 bp
cauliflower mosaic virus polyadenylation signal
CaMV poly(A) signal
9250 .. 9424  =  175 bp
cauliflower mosaic virus polyadenylation signal
bom
6928 .. 7068  =  141 bp
basis of mobility region from pBR322
bom
6928 .. 7068  =  141 bp
basis of mobility region from pBR322
lac promoter
11,461 .. 11,491  =  31 bp
   Segment 1:  -35  
   11,461 .. 11,466  =  6 bp
promoter for the E. coli lac operon
lac promoter
11,461 .. 11,491  =  31 bp
   Segment 2:  
   11,467 .. 11,484  =  18 bp
promoter for the E. coli lac operon
lac promoter
11,461 .. 11,491  =  31 bp
   Segment 3:  -10  
   11,485 .. 11,491  =  7 bp
promoter for the E. coli lac operon
lac promoter
11,461 .. 11,491  =  31 bp
3 segments
promoter for the E. coli lac operon
RB T-DNA repeat
2869 .. 2893  =  25 bp
right border repeat from nopaline C58 T-DNA
RB T-DNA repeat
2869 .. 2893  =  25 bp
right border repeat from nopaline C58 T-DNA
LB T-DNA repeat
9148 .. 9172  =  25 bp
left border repeat from nopaline C58 T-DNA
LB T-DNA repeat
9148 .. 9172  =  25 bp
left border repeat from nopaline C58 T-DNA
6xHis
2546 .. 2563  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
2546 .. 2563  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
lac operator
11,499 .. 11,515  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
11,499 .. 11,515  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
MCS
11,549 .. 11,605  =  57 bp
pUC18/19 multiple cloning site
MCS
11,549 .. 11,605  =  57 bp
pUC18/19 multiple cloning site
M13 rev
11,523 .. 11,539  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
11,523 .. 11,539  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
11,609 .. 11,625  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
11,609 .. 11,625  =  17 bp
common sequencing primer, one of multiple similar
variants
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter