Resources
Plasmid Files

pCAMBIA2300

Agrobacterium binary vector for plant transformation, with kanamycin-resistance genes.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pCAMBIA2300 Sequence and MappCAMBIA2300.dna
Map and Sequence File   
Sequence Author:  Cambia
Download Free Trial Get SnapGene Viewer

 PmeI (8623) HindIII (8410) PstI - SbfI (8402) SalI (8392) XbaI (8386) BamHI (8380) SmaI (8377) KpnI - TspMI - XmaI (8375) Acc65I (8371) SacI (8369) Eco53kI (8367) EcoRI (8359) lac operator BstXI (8116) BglII (7316) NcoI (7301) BssHII (6771) RsrII (6653) PspXI (6451) BclI * (5936) SacII (5933) PsiI (5805) Bpu10I (5660) EcoO109I - PpuMI (5521) BspHI (5479) BlpI (5329) SspI (4983) NsiI (4945) BsaBI * (1331) PasI (1338) AclI (1932) BsiWI (2892) NheI (3008) BmtI (3012) EcoNI (3321) BsaI (3411) AgeI (3502) MreI - SgrAI (3790) BstZ17I (3992) pCAMBIA2300 8743 bp
PmeI  (8623)
1 site
G T T T A A A C C A A A T T T G
HindIII  (8410)
1 site
A A G C T T T T C G A A
PstI  (8402)
1 site
C T G C A G G A C G T C
SbfI  (8402)
1 site
C C T G C A G G G G A C G T C C
SalI  (8392)
1 site
G T C G A C C A G C T G
XbaI  (8386)
1 site
T C T A G A A G A T C T
BamHI  (8380)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
SmaI  (8377)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
KpnI  (8375)
1 site
G G T A C C C C A T G G
TspMI  (8375)
1 site
C C C G G G G G G C C C
XmaI  (8375)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
Acc65I  (8371)
1 site
G G T A C C C C A T G G
SacI  (8369)
1 site
G A G C T C C T C G A G
Eco53kI  (8367)
1 site
G A G C T C C T C G A G
EcoRI  (8359)
1 site
G A A T T C C T T A A G
BstXI  (8116)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BglII  (7316)
1 site
A G A T C T T C T A G A
NcoI  (7301)
1 site
C C A T G G G G T A C C
BssHII  (6771)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
RsrII  (6653)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PspXI  (6451)
1 site
V C T C G A G B B G A G C T C V
BclI  (5936)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
SacII  (5933)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
PsiI  (5805)
1 site
T T A T A A A A T A T T
Bpu10I  (5660)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
EcoO109I  (5521)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PpuMI  (5521)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
BspHI  (5479)
1 site
T C A T G A A G T A C T
BlpI  (5329)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
SspI  (4983)
1 site
A A T A T T T T A T A A
NsiI  (4945)
1 site
A T G C A T T A C G T A
BsaBI  (1331)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
PasI  (1338)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
AclI  (1932)
1 site
A A C G T T T T G C A A
BsiWI  (2892)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
NheI  (3008)
1 site
G C T A G C C G A T C G
BmtI  (3012)
1 site
G C T A G C C G A T C G
EcoNI  (3321)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BsaI  (3411)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AgeI  (3502)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
MreI  (3790)
1 site
C G C C G G C G G C G G C C G C
SgrAI  (3790)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
BstZ17I  (3992)
1 site
G T A T A C C A T A T G
pVS1 RepA
2277 .. 3350  =  1074 bp
357 amino acids  =  39.9 kDa
Product: replication protein from Pseudomonas
plasmid pVS1
pVS1 RepA
2277 .. 3350  =  1074 bp
357 amino acids  =  39.9 kDa
Product: replication protein from Pseudomonas
plasmid pVS1
NeoR/KanR
6507 .. 7304  =  798 bp
265 amino acids  =  29.1 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin®)
NeoR/KanR
6507 .. 7304  =  798 bp
265 amino acids  =  29.1 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin®)
KanR
4955 .. 5749  =  795 bp
264 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
KanR
4955 .. 5749  =  795 bp
264 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
CaMV 35S promoter (enhanced)
7367 .. 8044  =  678 bp
cauliflower mosaic virus 35S promoter with a
duplicated enhancer region
CaMV 35S promoter (enhanced)
7367 .. 8044  =  678 bp
cauliflower mosaic virus 35S promoter with a
duplicated enhancer region
pVS1 StaA
1219 .. 1848  =  630 bp
209 amino acids  =  22.1 kDa
Product: stability protein from Pseudomonas
plasmid pVS1
pVS1 StaA
1219 .. 1848  =  630 bp
209 amino acids  =  22.1 kDa
Product: stability protein from Pseudomonas
plasmid pVS1
ori
4280 .. 4868  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
4280 .. 4868  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
lacZα
8345 .. 8578  =  234 bp
77 amino acids  =  8.6 kDa
Product: LacZα fragment of β-galactosidase
lacZα
8345 .. 8578  =  234 bp
77 amino acids  =  8.6 kDa
Product: LacZα fragment of β-galactosidase
pVS1 oriV
3416 .. 3610  =  195 bp
origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)
pVS1 oriV
3416 .. 3610  =  195 bp
origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)
CaMV poly(A) signal
6276 .. 6450  =  175 bp
cauliflower mosaic virus polyadenylation signal
CaMV poly(A) signal
6276 .. 6450  =  175 bp
cauliflower mosaic virus polyadenylation signal
bom
3954 .. 4094  =  141 bp
basis of mobility region from pBR322
bom
3954 .. 4094  =  141 bp
basis of mobility region from pBR322
lac promoter
8271 .. 8301  =  31 bp
   Segment 1:  -35  
   8271 .. 8276  =  6 bp
promoter for the E. coli lac operon
lac promoter
8271 .. 8301  =  31 bp
   Segment 2:  
   8277 .. 8294  =  18 bp
promoter for the E. coli lac operon
lac promoter
8271 .. 8301  =  31 bp
   Segment 3:  -10  
   8295 .. 8301  =  7 bp
promoter for the E. coli lac operon
lac promoter
8271 .. 8301  =  31 bp
3 segments
promoter for the E. coli lac operon
LB T-DNA repeat
6174 .. 6198  =  25 bp
left border repeat from nopaline C58 T-DNA
LB T-DNA repeat
6174 .. 6198  =  25 bp
left border repeat from nopaline C58 T-DNA
RB T-DNA repeat
8638 .. 8662  =  25 bp
right border repeat from nopaline C58 T-DNA
RB T-DNA repeat
8638 .. 8662  =  25 bp
right border repeat from nopaline C58 T-DNA
lac operator
8309 .. 8325  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
8309 .. 8325  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
MCS
8359 .. 8415  =  57 bp
pUC18/19 multiple cloning site
MCS
8359 .. 8415  =  57 bp
pUC18/19 multiple cloning site
M13 rev
8333 .. 8349  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
8333 .. 8349  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
8419 .. 8435  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
8419 .. 8435  =  17 bp
common sequencing primer, one of multiple similar
variants
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter