pEarleyGate 203

Gateway®-compatible plant transformation vector with an N-terminal Myc tag.

Sequence Author: Pikaard Lab

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LB T-DNA repeat PsiI (11,415) BspHI (11,089) EcoRV (10,666) PluTI (9407) SfoI (9405) NarI (9404) KasI (9403) EcoNI (8931) BmtI (8622) NheI (8618) BsiWI (8502) PmeI (5490) AseI (92) Acc65I (407) KpnI (411) FspAI (521) PaqCI (585) AfeI (758) BstBI (1770) StuI (1868) PshAI (2467) PaeR7I - PspXI - XhoI (2654) TMV Ω ATG Myc attR1 lac UV5 promoter BspEI (3215) BbvCI (3944) SrfI (4090) BstXI (4207) AvrII (4479) PacI (4526) SpeI (4538) MfeI (4842) BstEII (5114) AhdI (5244) SbfI (5269) HindIII (5277) PvuI (5403) pEarleyGate 203 11,783 bp
PsiI  (11,415)
1 site
T T A T A A A A T A T T
BspHI  (11,089)
1 site
T C A T G A A G T A C T
EcoRV  (10,666)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PluTI  (9407)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (9405)
1 site
G G C G C C C C G C G G
NarI  (9404)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (9403)
1 site
G G C G C C C C G C G G
EcoNI  (8931)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BmtI  (8622)
1 site
G C T A G C C G A T C G
NheI  (8618)
1 site
G C T A G C C G A T C G
BsiWI  (8502)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
PmeI  (5490)
1 site
G T T T A A A C C A A A T T T G
AseI  (92)
1 site
A T T A A T T A A T T A
Acc65I  (407)
1 site
G G T A C C C C A T G G
KpnI  (411)
1 site
G G T A C C C C A T G G
FspAI  (521)
1 site
R T G C G C A Y Y A C G C G T R
PaqCI  (585)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
AfeI  (758)
1 site
A G C G C T T C G C G A
BstBI  (1770)
1 site
T T C G A A A A G C T T
StuI  (1868)
1 site
A G G C C T T C C G G A
PshAI  (2467)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
PaeR7I  (2654)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (2654)
1 site
V C T C G A G B B G A G C T C V
XhoI  (2654)
1 site
C T C G A G G A G C T C
BspEI  (3215)
1 site
T C C G G A A G G C C T
BbvCI  (3944)
1 site
C C T C A G C G G A G T C G
SrfI  (4090)
1 site
G C C C G G G C C G G G C C C G
BstXI  (4207)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
AvrII  (4479)
1 site
C C T A G G G G A T C C
PacI  (4526)
1 site
T T A A T T A A A A T T A A T T
SpeI  (4538)
1 site
A C T A G T T G A T C A
MfeI  (4842)
1 site
C A A T T G G T T A A C
BstEII  (5114)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
AhdI  (5244)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
SbfI  (5269)
1 site
C C T G C A G G G G A C G T C C
HindIII  (5277)
1 site
A A G C T T T T C G A A
PvuI  (5403)
1 site
C G A T C G G C T A G C
pVS1 RepA
7887 .. 8960  =  1074 bp
357 amino acids  =  39.9 kDa
Product: replication protein from Pseudomonas plasmid pVS1
pVS1 RepA
7887 .. 8960  =  1074 bp
357 amino acids  =  39.9 kDa
Product: replication protein from Pseudomonas plasmid pVS1
KanR
10,565 .. 11,359  =  795 bp
264 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
KanR
10,565 .. 11,359  =  795 bp
264 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
OCS terminator
4554 .. 5261  =  708 bp
octopine synthase terminator
OCS terminator
4554 .. 5261  =  708 bp
octopine synthase terminator
CmR
3006 .. 3665  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
3006 .. 3665  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
pVS1 StaA
6829 .. 7458  =  630 bp
209 amino acids  =  22.1 kDa
Product: stability protein from Pseudomonas plasmid pVS1
pVS1 StaA
6829 .. 7458  =  630 bp
209 amino acids  =  22.1 kDa
Product: stability protein from Pseudomonas plasmid pVS1
ori
9890 .. 10,478  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
9890 .. 10,478  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
BlpR
373 .. 924  =  552 bp
183 amino acids  =  20.6 kDa
Product: phosphinothricin acetyltransferase
confers resistance to bialophos or phosphinothricin
BlpR
373 .. 924  =  552 bp
183 amino acids  =  20.6 kDa
Product: phosphinothricin acetyltransferase
confers resistance to bialophos or phosphinothricin
MAS promoter
930 .. 1310  =  381 bp
mannopine synthase promoter (Velten et al., 1984)
MAS promoter
930 .. 1310  =  381 bp
mannopine synthase promoter (Velten et al., 1984)
CaMV 35S promoter
2306 .. 2651  =  346 bp
strong constitutive promoter from cauliflower mosaic virus
CaMV 35S promoter
2306 .. 2651  =  346 bp
strong constitutive promoter from cauliflower mosaic virus
ccdB
4007 .. 4312  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA gyrase
Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains.
ccdB
4007 .. 4312  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA gyrase
Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains.
MAS terminator
111 .. 363  =  253 bp
mannopine synthase terminator
MAS terminator
111 .. 363  =  253 bp
mannopine synthase terminator
pVS1 oriV
9026 .. 9220  =  195 bp
origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)
pVS1 oriV
9026 .. 9220  =  195 bp
origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)
bom
9564 .. 9704  =  141 bp
basis of mobility region from pBR322
bom
9564 .. 9704  =  141 bp
basis of mobility region from pBR322
attR2
4353 .. 4477  =  125 bp
recombination site for the Gateway® LR reaction
attR2
4353 .. 4477  =  125 bp
recombination site for the Gateway® LR reaction
attR1
2773 .. 2892  =  120 bp
recombination site for the Gateway® LR reaction
attR1
2773 .. 2892  =  120 bp
recombination site for the Gateway® LR reaction
TMV Ω
2681 .. 2735  =  55 bp
translational enhancer from the tobacco mosaic virus 5'-leader sequence (Gallie et al., 1988)
TMV Ω
2681 .. 2735  =  55 bp
translational enhancer from the tobacco mosaic virus 5'-leader sequence (Gallie et al., 1988)
ATG
2737 .. 2739  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
2737 .. 2739  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
Myc
2740 .. 2769  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc oncogene) epitope tag
Myc
2740 .. 2769  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc oncogene) epitope tag
lac UV5 promoter
2922 .. 2952  =  31 bp
3 segments
   Segment 1:  -35  
   2922 .. 2927  =  6 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
2922 .. 2952  =  31 bp
3 segments
   Segment 2:  
   2928 .. 2945  =  18 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
2922 .. 2952  =  31 bp
3 segments
   Segment 3:  -10  
   2946 .. 2952  =  7 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
2922 .. 2952  =  31 bp
3 segments
E. coli lac promoter with an "up" mutation
LB T-DNA repeat
1 .. 25  =  25 bp
left border repeat from nopaline C58 T-DNA
LB T-DNA repeat
1 .. 25  =  25 bp
left border repeat from nopaline C58 T-DNA
RB T-DNA repeat
5505 .. 5529  =  25 bp
right border repeat from nopaline C58 T-DNA
RB T-DNA repeat
5505 .. 5529  =  25 bp
right border repeat from nopaline C58 T-DNA
ORF:  448 .. 957  =  510 bp
ORF:  169 amino acids  =  18.0 kDa
ORF:  6829 .. 7458  =  630 bp
ORF:  209 amino acids  =  22.1 kDa
ORF:  275 .. 607  =  333 bp
ORF:  110 amino acids  =  11.9 kDa
ORF:  1730 .. 2134  =  405 bp
ORF:  134 amino acids  =  15.5 kDa
ORF:  4007 .. 4312  =  306 bp
ORF:  101 amino acids  =  11.7 kDa
ORF:  5141 .. 5431  =  291 bp
ORF:  96 amino acids  =  10.1 kDa
ORF:  1809 .. 2072  =  264 bp
ORF:  87 amino acids  =  10.4 kDa
ORF:  2124 .. 2552  =  429 bp
ORF:  142 amino acids  =  15.9 kDa
ORF:  3006 .. 3665  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  5844 .. 6530  =  687 bp
ORF:  228 amino acids  =  25.1 kDa
ORF:  7785 .. 8960  =  1176 bp
ORF:  391 amino acids  =  43.9 kDa
ORF:  10,698 .. 10,928  =  231 bp
ORF:  76 amino acids  =  8.3 kDa
ORF:  6567 .. 6881  =  315 bp
ORF:  104 amino acids  =  12.0 kDa
ORF:  7755 .. 8327  =  573 bp
ORF:  190 amino acids  =  20.6 kDa
ORF:  1757 .. 2125  =  369 bp
ORF:  122 amino acids  =  14.2 kDa
ORF:  5876 .. 6226  =  351 bp
ORF:  116 amino acids  =  12.6 kDa
ORF:  7733 .. 7990  =  258 bp
ORF:  85 amino acids  =  9.6 kDa
ORF:  10,565 .. 11,359  =  795 bp
ORF:  264 amino acids  =  31.0 kDa
ORF:  11,604 .. 321  =  501 bp
ORF:  166 amino acids  =  18.5 kDa
ORF:  373 .. 924  =  552 bp
ORF:  183 amino acids  =  20.6 kDa
ORF:  6295 .. 6567  =  273 bp
ORF:  90 amino acids  =  9.9 kDa
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