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pGreen

Compact Agrobacterium binary vector with a kanamycin-resistance gene. Also known as pGreen 0000.

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pGreen.dna
Map and Sequence File:    Download    Open   
Sequence Author:  pGreen website
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BsaBI * (3199) DraIII (3011) BsrDI (2987) BspHI (2955) NruI (2820) PasI (2602) EcoNI (2564) SspI (2552) AsiSI (2479) Bpu10I - BsmBI (2457) PflMI (2217) AcuI (1892) AlwNI (1760) ApaLI (1658) PspFI (1652) BseYI (1648) StyI (330) NgoMIV (433) NaeI (435) FseI (437) AanI - AanI - PsiI (455) BglII (534) HpaI (566) FspI (606) BmrI (707) Acc65I (777) KpnI (781) PspOMI (783) EcoO109I (784) ApaI (787) AbsI - PaeR7I - PspXI - XhoI (792) SalI (798) AccI (799) BspDI - ClaI (808) HindIII (813) EcoRV (821) EcoRI (825) PstI (835) TspMI - XmaI (837) SmaI (839) BamHI (843) SpeI (849) XbaI (855) NotI (862) AleI - MslI (873) SacII (874) BstXI (875) Eco53kI (881) SacI (883) BspQI - SapI (1162) StuI (1286) BglII (1339) DrdI (1452) BciVI (1547) pGreen 3228 bp
BsaBI  (3199)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
DraIII  (3011)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BsrDI  (2987)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
BspHI  (2955)
1 site
T C A T G A A G T A C T
NruI  (2820)
1 site
T C G C G A A G C G C T
PasI  (2602)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
EcoNI  (2564)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
SspI  (2552)
1 site
A A T A T T T T A T A A
AsiSI  (2479)
1 site
G C G A T C G C C G C T A G C G
Bpu10I  (2457)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BsmBI  (2457)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
PflMI  (2217)
1 site
C