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pPZP111

Agrobacterium binary vector for plant transformation, with chloramphenicol- and kanamycin-resistance genes. The MCS is reversed in pPZP112.

 
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pPZP111.dna
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PmeI (8758) HindIII (8545) PstI - SbfI (8537) SalI (8527) XbaI (8521) BamHI (8515) SmaI (8512) KpnI - TspMI - XmaI (8510) Acc65I (8506) SacI (8504) Eco53kI (8502) EcoRI (8494) BstXI (8251) EcoRV (7599) BglII (7453) NcoI (7438) BssHII (6908) RsrII (6790) PmlI (6522) BclI * (6056) BspEI (5552) SspI (5240) HpaI (5033) NsiI (4913) BsiWI (2860) NheI (2976) BmtI (2980) EcoNI (3289) BsaI (3379) AgeI (3470) BstZ17I (3960) pPZP111 8910 bp
PmeI  (8758)
1 site
G T T T A A A C C A A A T T T G
HindIII  (8545)
1 site
A A G C T T T T C G A A
PstI  (8537)
1 site
C T G C A G G A C G T C
SbfI  (8537)
1 site
C C T G C A G G G G A C G T C C
SalI  (8527)
1 site
G T C G A C C A G C T G
XbaI  (8521)
1 site
T C T A G A A G A T C T
BamHI  (8515)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SmaI  (8512)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
KpnI  (8510)
1 site
G G T A C C C C A T G