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pPZP112

Agrobacterium binary vector for plant transformation, with chloramphenicol- and kanamycin-resistance genes. The MCS is reversed in pPZP111.

 
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pPZP112.dna
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PmeI (8758) EcoRI (8479) SacI (8477) Eco53kI (8475) KpnI (8471) Acc65I (8467) SmaI (8465) TspMI - XmaI (8463) BamHI (8458) XbaI (8452) SalI (8446) PstI - SbfI (8444) HindIII (8428) BstXI (8251) EcoRV (7599) BglII (7453) NcoI (7438) BssHII (6908) RsrII (6790) PmlI (6522) BclI * (6056) BspEI (5552) SspI (5240) HpaI (5033) NsiI (4913) BsiWI (2860) NheI (2976) BmtI (2980) EcoNI (3289) BsaI (3379) AgeI (3470) BstZ17I (3960) pPZP112 8910 bp
PmeI  (8758)
1 site
G T T T A A A C C A A A T T T G
EcoRI  (8479)
1 site
G A A T T C C T T A A G
SacI  (8477)
1 site
G A G C T C C T C G A G
Eco53kI  (8475)
1 site
G A G C T C C T C G A G
KpnI  (8471)
1 site
G G T A C C C C A T G G
Acc65I  (8467)
1 site
G G T A C C C C A T G G
SmaI  (8465)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (8463)
1 site
C C C G G G G G G C C C
XmaI  (8463)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
BamHI  (8458)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (8452)
1 site
T C T A G A A G A T C T
SalI  (8446)
1 site
G T C G A C C A G C T G
PstI  (8444)
1 site
C T G C A G G A C G T C
SbfI  (8444)
1 site
C C T G C A G G G G A C G T C C
HindIII  (8428)
1 site
A A G C T T T T C G A A
BstXI  (8251)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
EcoRV  (7599)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BglII  (7453)
1 site
A G A T C T T C T A G A
NcoI  (7438)
1 site
C C A T G G G G T A C C
BssHII  (6908)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
RsrII  (6790)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PmlI  (6522)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
BclI  (6056)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BspEI  (5552)
1 site