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pPZP212

Agrobacterium binary vector for plant transformation, with spectinomycin- and kanamycin-resistance genes. The MCS is reversed in pPZP211.

 
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pPZP212.dna
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PmeI (8863) EcoRI (8584) SacI (8582) Eco53kI (8580) KpnI (8576) Acc65I (8572) SmaI (8570) TspMI - XmaI (8568) BamHI (8563) XbaI (8557) SalI (8551) PstI - SbfI (8549) HindIII (8533) BstXI (8356) EcoRV (7704) BglII (7558) NcoI (7543) RsrII (6895) PmlI (6627) BstEII (5707) BlpI (5560) HpaI (5033) NsiI (4913) PasI (1306) AclI (1900) BsiWI (2860) NheI (2976) BmtI (2980) EcoNI (3289) BsaI (3379) AgeI (3470) BstZ17I (3960) pPZP212 9015 bp
PmeI  (8863)
1 site
G T T T A A A C C A A A T T T G
EcoRI  (8584)
1 site
G A A T T C C T T A A G
SacI  (8582)
1 site
G A G C T C C T C G A G
Eco53kI  (8580)
1 site
G A G C T C C T C G A G
KpnI  (8576)
1 site
G G T A C C C C A T G G
Acc65I  (8572)
1 site
G G T A C C C C A T G G
SmaI  (8570)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (8568)
1 site
C C C G G G G G G C C C
XmaI  (8568)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
BamHI  (8563)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (8557)
1 site
T C T A G A A G A T C T
SalI  (8551)
1 site
G T C G A C C A G C T G
PstI  (8549)
1 site
C T G C A G G A C G T C
SbfI  (8549)
1 site
C C T G C A G G G G A C G T C C
HindIII  (8533)
1 site
A A G C T T T T C G A A
BstXI  (8356)
1 site
C C A N N N N N N T