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pPZP222

Agrobacterium binary vector for plant transformation, with spectinomycin- and gentamycin-resistance genes. The MCS is reversed in pPZP221.

 
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pPZP222.dna
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PmeI (8580) EcoRI (8301) BanII - SacI (8299) Eco53kI (8297) KpnI (8293) Acc65I (8289) SmaI (8287) TspMI - XmaI (8285) BamHI (8280) XbaI (8274) SalI (8268) PstI - SbfI (8266) HindIII (8250) BstXI (8073) NcoI (7260) PmlI (6627) BstEII (5707) BpmI (5622) BlpI (5560) BssHII (5144) HpaI (5033) NsiI (4913) PasI (1306) AclI (1900) BsiWI (2860) NheI (2976) BmtI (2980) BspDI * - ClaI * (3056) EcoNI (3289) BsaI (3379) AgeI (3470) KasI (3761) NarI (3762) SfoI (3763) PluTI (3765) BstZ17I (3960) pPZP222 8732 bp
PmeI  (8580)
1 site
G T T T A A A C C A A A T T T G
EcoRI  (8301)
1 site
G A A T T C C T T A A G
BanII  (8299)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
SacI  (8299)
1 site
G A G C T C C T C G A G
Eco53kI  (8297)
1 site
G A G C T C C T C G A G
KpnI  (8293)
1 site