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Plasmid Files

pRI 910

Agrobacterium binary vector with a multiple cloning site (MCS), for plant cell transformation. The MCS is reversed in pRI 909.

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pRI 910.dna
Map and Sequence File:    Download    Open   
Sequence Author:  TaKaRa
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PpuMI - Psp5II - PspPPI (8573) MssI - PmeI (7884) HindIII (7759) BbuI - PaeI - SphI (7757) PstI - SbfI - SdaI - Sse8387I (7751) AccI - FblI - XmiI (7742) SalI (7741) XbaI (7735) BamHI (7729) SmaI (7726) Cfr9I - KpnI - TspMI - XmaI - XmaCI (7724) Acc65I - Asp718I (7720) Psp124BI - SacI - SstI (7718) Ecl136II - Eco53kI - EcoICRI (7716) EcoRI (7708) Acc16I - AviII - FspI - NsbI (6982) BsaAI - BstBAI - Ppu21I (6780) ApaI (6151) Bsp120I - PspOMI (6147) BspLU11I - PciI - PscI (6031) EcoT22I - Mph1103I - NsiI - Zsp2I (6028) MauBI (5916) TatI (5602) PspXI (5493) BbvCI (5292) AspA2I - AvrII - BlnI - XmaJI (5073) Alw44I - ApaLI - VneI (310) MfeI - MunI (1473) BstEII - BstPI - Eco91I - EcoO65I - PspEI (1838) BstAPI (2018) AccB7I - PflMI - Van91I (2086) BclI * - FbaI * - Ksp22I * (2394) AjiI - BmgBI - BtrI (3159) AccIII - Aor13HI - BseAI - Bsp13I - BspEI - Kpn2I - MroI (3940) AgeI - AsiGI - BshTI - CspAI - PinAI (4300) pRI 910 9168 bp
PpuMI  (8573)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
Psp5II  (8573)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different Psp5II sites may not be compatible.
PspPPI  (8573)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PspPPI sites may not be compatible.
MssI  (7884)
1 site
G T T T A A A C C A A A T T T G
PmeI  (7884)
1 site
G T T T A A A C C A A A T T T G
HindIII  (7759)
1 site
A A G C T T T T C G A A
BbuI  (7757)
1 site
G C A T G C C G T A C G
PaeI  (7757)
1 site
G C A T G C C G T A C G
SphI  (7757)
1 site
G C A T G C C G T A C G
PstI  (7751)
1 site
C T G C A G G A C G T C
SbfI  (7751)
1 site
C C T G C A G G G G A C G T C C
SdaI  (7751)
1 site
C C T G C A G G G G A C G T C C
Sse8387I  (7751)
1 site
C C T G C A G G G G A C G T C C
AccI  (7742)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
FblI  (7742)
1 site
G T M K A C C A K M T G

Sticky ends from different FblI sites may not be compatible.
XmiI  (7742)
1 site
G T M K A C C A K M T G

Sticky ends from different XmiI sites may not be compatible.
SalI  (7741)
1 site
G T C G A C C A G C T G
XbaI  (7735)
1 site
T C T A G A A G A T C T
BamHI  (7729)
1 site
G G A T C C C C T A G G