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pQE-100 DoubleTag™

Bacterial vector for expressing proteins with an N-terminal 6xHis tag and a C-terminal Tag-100 tag.

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pQE-100 DoubleTag.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Qiagen
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BbsI (3498) EcoO109I (3496) AatII (3442) ZraI (3440) XmnI (3119) PvuI (2890) FspI (2742) AseI (2692) NmeAIII (2668) BglI (2640) BsrFI (2600) BsaI (2581) AhdI (2520) AlwNI (2043) PspFI (1935) BseYI (1931) PaeR7I - XhoI (1) PsiI (49) MfeI (59) EcoRI (88) BseRI (115) BamHI (145) SphI (155) Eco53kI (159) BanII - SacI (161) Acc65I (163) KpnI (167) TspMI - XmaI (168) SmaI (170) SalI (173) HincII (175) PstI (183) BsgI (185) BfuAI - BspMI (186) BglII (232) BlpI (250) NheI (358) BmtI (362) Bpu10I (383) PvuII (510) BspEI (606) PasI - PflMI * (842) MscI (877) BtgI - NcoI - StyI (911) XbaI (1214) PfoI (1271) PflFI - Tth111I (1374) BsaAI (1381) BstZ17I (1400) NdeI (1450) BstAPI (1451) BspQI - SapI (1511) AflIII - PciI (1627) pQE-100 DoubleTag™ 3512 bp
BbsI  (3498)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
EcoO109I  (3496)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
AatII  (3442)
1 site
G A C G T C C T G C A G
ZraI  (3440)
1 site
G A C G T C C T G C A G
XmnI  (3119)
1 site
G A A N N N N T T C C T T N N N N A A G
PvuI  (2890)
1 site
C G A T C G G C T A G C
FspI  (2742)
1 site
T G C G C A A C G C G T
AseI  (2692)
1 site
A T T A A T T A A T T A
NmeAIII  (2668)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (2640)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsrFI  (2600)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BsaI  (2581)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (2520)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2043)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (1935)
1 site
C C C A G C G G G T C G
BseYI  (1931)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
PaeR7I  (1)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1)
1 site
C T C G A G G A G C T C
PsiI  (49)
1 site
T T A T A A A A T A T T
MfeI  (59)
1 site
C A A T T G G T T A A C
EcoRI  (88)
1 site
G A A T T C C T T A A G
BseRI  (115)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BamHI  (145)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SphI  (155)
1 site
G C A T G C C G T A C G