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Plasmid Files

pQE-11

Bacterial vector for expressing N-terminally 6xHis-tagged proteins. For other reading frames, use pQE‑9 or pQE‑10.

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pQE-11.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Qiagen
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BbsI (3427) EcoO109I (3425) AatII (3371) ZraI (3369) XmnI (3048) PvuI (2819) FspI (2671) AseI (2621) NmeAIII (2597) BglI (2569) BsrFI (2529) BsaI (2510) AhdI (2449) AlwNI (1972) PspFI (1864) BseYI (1860) AvaI - BsoBI - PaeR7I - XhoI (1) BmeT110I (2) PsiI (49) MfeI (59) EcoRI (88) BseRI (115) BstXI (142) BamHI (146) SalI (152) HincII (154) PstI (162) BfuAI - BspMI (165) HindIII (166) BlpI (178) NheI (286) BmtI (290) Bpu10I (311) PvuII (438) BspEI (534) PasI - PflMI * (770) MscI (805) XbaI (1143) PfoI (1200) PflFI - Tth111I (1303) BsaAI (1310) BstZ17I (1329) NdeI (1379) BstAPI (1380) BspQI - SapI (1440) AflIII - PciI (1556) pQE-11 3441 bp
BbsI  (3427)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
EcoO109I  (3425)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
AatII  (3371)
1 site
G A C G T C C T G C A G
ZraI  (3369)
1 site
G A C G T C C T G C A G
XmnI  (3048)
1 site
G A A N N N N T T C C T T N N N N A A G
PvuI  (2819)
1 site
C G A T C G G C T A G C
FspI  (2671)
1 site
T G C G C A A C G C G T
AseI  (2621)
1 site
A T T A A T T A A T T A
NmeAIII  (2597)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (2569)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsrFI  (2529)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BsaI  (2510)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (2449)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (1972)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (1864)
1 site
C C C A G C G G G T C G
BseYI  (1860)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
AvaI  (1)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (1)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (1)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1)
1 site
C T C G A G G A G C T C
BmeT110I  (2)
1 site
C Y C G R G G R G C Y C
PsiI  (49)
1 site
T T A T A A A A T A T T
MfeI  (59)
1 site
C A A T T G G T T A A C
EcoRI  (88)
1 site
G A A T T C C T T A A G
BseRI  (115)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BstXI  (142)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BamHI  (146)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SalI  (152)
1 site
G T C G A C C A G C T G
HincII  (154)
1 site
G T Y R A C C A R Y T G
PstI  (162)
1 site
C T G C A G G A C G T C
BfuAI  (165)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (165)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
HindIII  (166)
1 site
A A G C T T T T C G A A
BlpI  (178)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
NheI  (286)
1 site
G C T A G C C G A T C G
BmtI  (290)
1 site
G C T A G C C G A T C G
Bpu10I  (311)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
PvuII  (438)
1 site
C A G C T G G T C G A C
BspEI  (534)
1 site
T C C G G A A G G C C T
PasI  (770)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
PflMI  (770)
1 site
C C A N N N N N T G G G G T N N N N N A C C