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Plasmid Files

pQE-T7 (cloning region)

Cloning region of pQE-T7, the parental vector for the QIAgenes system.

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pQE-T7 (cloning region) Sequence and MappQE-T7 (cloning region).dna
Map and Sequence File   
Sequence Author:  Qiagen
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 250 200 150 100 50 T7 promoter lac operator ATG 6xHis QIAgene start codon stop codons 6xHis T7 terminator End (276) EcoO109I (245) StyI (240) BlpI (218) MspA1I (217) EcoP15I (178) BstYI (165) BsiHKAI - Bsp1286I (146) BmeT110I (140) AvaI - BsoBI - PaeR7I - PspXI - XhoI (139) NdeI (126) NdeI (102) XbaI (62) BsrBI (45) SfcI (28) AseI (15) Start (0) pQE-T7 (cloning region) 276 bp
End  (276)
0 sites
EcoO109I  (245)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
StyI  (240)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BlpI  (218)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
MspA1I  (217)
1 site
C M G C K G G K C G M C
EcoP15I  (178)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the
EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
BstYI  (165)
1 site
R G A T C Y Y C T A G R
BsiHKAI  (146)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
Bsp1286I  (146)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
BmeT110I  (140)
1 site
C Y C G R G G R G C Y C
AvaI  (139)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (139)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
PaeR7I  (139)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (139)
1 site
V C T C G A G B B G A G C T C V
XhoI  (139)
1 site
C T C G A G G A G C T C
NdeI  (126)
2 sites
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
NdeI  (102)
2 sites
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
XbaI  (62)
1 site
T C T A G A A G A T C T
BsrBI  (45)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures
up to 50°C.
SfcI  (28)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
AseI  (15)
1 site
A T T A A T T A A T T A
Start  (0)
0 sites
T7 promoter
16 .. 34  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
16 .. 34  =  19 bp
promoter for bacteriophage T7 RNA polymerase
lac operator
35 .. 59  =  25 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
35 .. 59  =  25 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
ATG
104 .. 106  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
104 .. 106  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
6xHis
110 .. 127  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
110 .. 127  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
QIAgene start codon
128 .. 130  =  3 bp
1 amino acid  =  149.2 Da
QIAgene start codon
128 .. 130  =  3 bp
1 amino acid  =  149.2 Da
stop codons
131 .. 136  =  6 bp
two tandem stop codons
stop codons
131 .. 136  =  6 bp
two tandem stop codons
6xHis
145 .. 162  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
145 .. 162  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
T7 terminator
229 .. 276  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
T7 terminator
229 .. 276  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
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