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Plasmid Files

p11

Bacterial vector for expressing a protein with an N-terminal 6xHis-TEV cassette.

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p11.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Midwest Center for Structural Genomics
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EcoRI (5702) BspDI - ClaI (5678) HindIII (5671) BlpI (5434) SpeI (5427) BamHI (5407) BmtI (5390) NheI (5386) NdeI (5381) NcoI (5315) XbaI (5276) BglII (5210) SgrAI (5169) SphI (5021) EcoNI (4956) MluI (4488) BclI * (4474) BstEII (4306) ApaI (4285) PspOMI (4281) BssHII (4077) HpaI (3986) PshAI (3647) EagI (3420) NruI (3389) BfuAI - BspMI (3305) BsmI (3006) BmeT110I (2935) AvaI - BsoBI (2934) MscI * (2917) ZraI (75) AatII (77) SspI (191) ScaI (515) PvuI (627) PstI (754) BsaI (930) AhdI (996) AlwNI (1475) PciI (1884) BspQI - SapI (2001) AccI (2116) BstZ17I (2117) BsaAI (2136) PflFI - Tth111I (2142) Bpu10I (2779) FspAI (2907) p11 5704 bp
EcoRI  (5702)
1 site
G A A T T C C T T A A G
BspDI  (5678)
1 site
A T C G A T T A G C T A
ClaI  (5678)
1 site
A T C G A T T A G C T A
HindIII  (5671)
1 site
A A G C T T T T C G A A
BlpI  (5434)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
SpeI  (5427)
1 site
A C T A G T T G A T C A
BamHI  (5407)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BmtI  (5390)
1 site
G C T A G C C G A T C G
NheI  (5386)
1 site
G C T A G C C G A T C G
NdeI  (5381)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
NcoI  (5315)
1 site
C C A T G G G G T A C C
XbaI  (5276)
1 site
T C T A G A A G A T C T
BglII  (5210)
1 site
A G A T C T T C T A G A
SgrAI  (5169)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
SphI  (5021)
1 site
G C A T G C C G T A C G
EcoNI  (4956)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
MluI  (4488)
1 site
A C G C G T T G C G C A
BclI  (4474)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BstEII  (4306)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
ApaI  (4285)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (4281)
1 site
G G G C C C C C C G G G
BssHII  (4077)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
HpaI  (3986)
1 site
G T T A A C C A A T T G
PshAI  (3647)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
EagI  (3420)
1 site
C G G C C G G C C G G C
NruI  (3389)
1 site
T C G C G A A G C G C T
BfuAI  (3305)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (3305)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BsmI  (3006)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BmeT110I  (2935)
1 site
C Y C G R G G R G C Y C
AvaI  (2934)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (2934)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
MscI  (2917)
1 site
T G G C C A A C C G G T
* Blocked by Dcm methylation.
ZraI  (75)
1 site
G A C G T C C T G C A G
AatII  (77)
1 site
G A C