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pBEN1-SGC

Bacterial vector encoding an N-terminal SET1-SBP-TEV cassette plus a SacB negative selection marker, for purification of recombinant proteins.

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pBEN1-SGC.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Structural Genomics Consortium
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XbaI (7481) BglII (7415) SgrAI (7374) SphI (7226) BstAPI (7017) MluI (6693) BclI * (6679) BstEII (6511) NmeAIII (6493) ApaI (6490) PspOMI (6486) BssHII (6282) FspI - FspAI (5615) PpuMI (5587) PflFI - Tth111I (4850) BspQI - SapI (4709) AlwNI (4183) NruI (3737) NdeI (6) SpeI (137) PmlI (175) Bsu36I (234) BsaI (302) NcoI (303) ZraI (316) AatII (318) BfuAI - BspMI (336) StuI * (1007) MscI (1029) AanI - AanI (1406) BsrGI (1446) SnaBI (1515) SacII (1845) BsaI (2233) BamHI (2249) EcoRI (2255) Eco53kI (2263) SacI (2265) SalI (2268) HindIII (2274) EagI - NotI (2281) PaeR7I - PspXI - XhoI (2289) BlpI (2368) AanI - AanI (2821) AsiSI - PvuI (3396) TspMI - XmaI (3518) SmaI (3520) pBEN1-SGC 7515 bp
XbaI  (7481)
1 site
T C T A G A A G A T C T
BglII  (7415)
1 site
A G A T C T T C T A G A
SgrAI  (7374)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
SphI  (7226)
1 site
G C A T G C C G T A C G
BstAPI  (7017)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
MluI  (6693)
1 site
A C G C G T T G C G C A
BclI  (6679)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BstEII  (6511)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
NmeAIII  (6493)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
ApaI  (6490)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (6486)
1 site
G G G C C C C C C G G G
BssHII  (6282)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
FspI  (5615)
1 site
T G C G C A A C G C G T
FspAI  (5615)
1 site
R T G C G C A Y Y A C G C G T R
PpuMI  (5587)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
PflFI  (4850)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4850)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BspQI  (4709)
1 site
G