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Plasmid Files

pMCSG31 (linearized)

Linearized bacterial vector for ligation-independent cloning (LIC), with an MBP-TVMV-6xHis-TEV leader and a Tet-inducible TVMV protease cassette.

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pMCSG31 (linearized).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Midwest Center for Structural Genomics
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PflMI (3274) BsgI (2397) AleI (2373) NruI (2115) SnaBI (2072) EcoNI (2043) Bpu10I (1996) AhdI (1752) BpmI (1683) NmeAIII (1605) MfeI (1574) FspI (1529) ScaI (1271) XmnI (1152) BstBI (279) SbfI (240) AbsI (224) SmaI (215) BseRI (3513) AgeI (3678) BsmBI (3819) BbsI (3863) AflIII - MluI (4177) SphI (4298) SgrAI (4446) BspDI * - ClaI * (4490) PfoI * (4855) BsiWI (4889) BglII (4958) BstXI (4984) BssHII (5035) BmgBI (5141) BsrGI (5716) Acc65I (5793) KpnI (5797) End (5807) Start (0) Eco53kI (31) SacI (33) EagI - NotI (49) TspMI - XmaI (213) pMCSG31 5807 bp