Plasmid Files |
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![]() | pMCSG50.dna |
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Sticky ends from different StyI sites may not be compatible. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different BspQI sites may not be compatible. |
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Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
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The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
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The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
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Sticky ends from different PpuMI sites may not be compatible. |
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Sticky ends from different BlpI sites may not be compatible. |
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Sticky ends from different AvaI sites may not be compatible. |
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Sticky ends from different BsoBI sites may not be compatible.BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Sticky ends from different BsmI sites may not be compatible. |
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Sticky ends from different DraIII sites may not be compatible. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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* Blocked by Dam methylation. |
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* Blocked by Dam methylation. |
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Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
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The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
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Sticky ends from different PflMI sites may not be compatible. |
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Sticky ends from different BstAPI sites may not be compatible. |
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* Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
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Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
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ApaI can be used between 25°C and 37°C. |
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BssHII is typically used at 50°C, but is 75% active at 37°C. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations. |
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lacI 1828 .. 2910 = 1083 bp 360 amino acids = 38.6 kDa Product: lac repressor The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
lacI 1828 .. 2910 = 1083 bp 360 amino acids = 38.6 kDa Product: lac repressor The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
BirA 241 .. 1206 = 966 bp 321 amino acids = 35.3 kDa Product: E. coli biotin ligase |
BirA 241 .. 1206 = 966 bp 321 amino acids = 35.3 kDa Product: E. coli biotin ligase |
AmpR 5099 .. 5959 = 861 bp 286 amino acids = 31.5 kDa Segment 2: 5099 .. 5890 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 5099 .. 5959 = 861 bp 286 amino acids = 31.5 kDa Segment 1: signal sequence 5891 .. 5959 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 5099 .. 5959 = 861 bp 286 amino acids = 31.5 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
ori 4340 .. 4928 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 4340 .. 4928 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
rop 3719 .. 3910 = 192 bp 63 amino acids = 7.2 kDa Product: Rop protein, which maintains plasmids at low copy number |
rop 3719 .. 3910 = 192 bp 63 amino acids = 7.2 kDa Product: Rop protein, which maintains plasmids at low copy number |
AmpR promoter 5960 .. 6063 = 104 bp |
AmpR promoter 5960 .. 6063 = 104 bp |
lacI promoter 1750 .. 1827 = 78 bp |
lacI promoter 1750 .. 1827 = 78 bp |
T7 terminator 26 .. 73 = 48 bp transcription terminator for bacteriophage T7 RNA polymerase |
T7 terminator 26 .. 73 = 48 bp transcription terminator for bacteriophage T7 RNA polymerase |
AviTag™ 1278 .. 1322 = 45 bp 15 amino acids = 1.8 kDa Product: peptide tag that allows for enzymatic biotinylation |
AviTag™ 1278 .. 1322 = 45 bp 15 amino acids = 1.8 kDa Product: peptide tag that allows for enzymatic biotinylation |
lac operator 1394 .. 1418 = 25 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
lac operator 1394 .. 1418 = 25 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
TEV site 1251 .. 1271 = 21 bp 7 amino acids = 900.0 Da Product: tobacco etch virus (TEV) protease recognition and cleavage site |
TEV site 1251 .. 1271 = 21 bp 7 amino acids = 900.0 Da Product: tobacco etch virus (TEV) protease recognition and cleavage site |
T7 promoter 1419 .. 1437 = 19 bp promoter for bacteriophage T7 RNA polymerase |
T7 promoter 1419 .. 1437 = 19 bp promoter for bacteriophage T7 RNA polymerase |
6xHis 140 .. 157 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
6xHis 140 .. 157 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
6xHis 1329 .. 1346 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
6xHis 1329 .. 1346 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
RBS 1215 .. 1220 = 6 bp ribosome binding site |
RBS 1215 .. 1220 = 6 bp ribosome binding site |
RBS 1358 .. 1363 = 6 bp ribosome binding site |
RBS 1358 .. 1363 = 6 bp ribosome binding site |
ATG 1347 .. 1349 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
ATG 1347 .. 1349 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
ORF: 1951 .. 2910 = 960 bp ORF: 319 amino acids = 34.1 kDa |
ORF: 2966 .. 3322 = 357 bp ORF: 118 amino acids = 13.0 kDa |
ORF: 3686 .. 3910 = 225 bp ORF: 74 amino acids = 8.5 kDa |
ORF: 1281 .. 2078 = 798 bp ORF: 265 amino acids = 28.3 kDa |
ORF: 2667 .. 2930 = 264 bp ORF: 87 amino acids = 8.9 kDa |
ORF: 5229 .. 5495 = 267 bp ORF: 88 amino acids = 9.2 kDa |
ORF: 1401 .. 1652 = 252 bp ORF: 83 amino acids = 8.6 kDa |
ORF: 1526 .. 1789 = 264 bp ORF: 87 amino acids = 9.5 kDa |
ORF: 2693 .. 2944 = 252 bp ORF: 83 amino acids = 9.1 kDa |
ORF: 5099 .. 5959 = 861 bp ORF: 286 amino acids = 31.5 kDa |
ORF: 241 .. 1206 = 966 bp ORF: 321 amino acids = 35.3 kDa |
ORF: 3319 .. 3687 = 369 bp ORF: 122 amino acids = 14.2 kDa |
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