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pMCSG53

Compact bacterial vector encoding tRNA genes for rare Arg and Ile codons, with a 6xHis-TEV leader for high-throughput purification of recombinant proteins.

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pMCSG53.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Midwest Center for Structural Genomics
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AccIII - Aor13HI - BseAI - Bsp13I - BspEI - Kpn2I - MroI (2) Acc113I - BmcAI - ScaI - ZrmI (4113) BpvUI - Ple19I - PvuI (4003) Acc16I - AviII - FspI - NsbI (3855) BglI (3753) BsaI - Bso31I - BspTNI - Eco31I (3694) AhdI - AspEI - BmeRI - DriI - Eam1105I (3633) AlwNI - CaiI (3156) AasI - DrdI - DseDI (2848) BspLU11I - PciI - PscI (2740) BspQI - LguI - PciSI - SapI (2624) BssNAI - Bst1107I - BstZ17I (2511) BsaAI - BstBAI - Ppu21I (2492) BoxI - BstPAI - PshAI (2485) BlpI - Bpu1102I - Bsp1720I - CelII (80) Ama87I - AvaI - BsiHKCI - BsoBI - Eco88I - PaeR7I - PspXI - Sfr274I - SlaI - XhoI (158) BmeT110I (159) BseX3I - BstZI - CciNI - EagI - EclXI - Eco52I - NotI (166) HindIII (173) SalI (179) Ecl136II - Eco53kI - EcoICRI (188) Psp124BI - SacI - SstI (190) EcoRI (192) BamHI (198) SspI (219) Acc65I - Asp718I (244) KpnI (248) BglII (251) FauNDI - NdeI (287) XbaI (325) BanIII * - Bsa29I * - BseCI * - BshVI - BspDI * - BspXI * - Bsu15I * - BsuTUI * - ClaI * (394) AgeI - AsiGI - BshTI - CspAI - PinAI (436) AscI - PalAI - SgsI (653) SbfI - SdaI - Sse8387I (664) AsuII - Bpu14I - Bsp119I - BspT104I - BstBI - Csp45I - NspV - SfuI (669) Acc36I - BfuAI - BspMI - BveI (682) MreI (801) AfeI - Aor51HI - Eco47III (887) Bsp19I - NcoI (1071) AanI - PsiI (1086) BstENI - EcoNI - XagI (1175) PfoI (1207) AccB7I - PflMI - Van91I (1222) BstAPI (1323) MluI (1640) BclI * - FbaI * - Ksp22I * (1654) BstEII - BstPI - Eco91I - EcoO65I - PspEI (1821) Bsp120I - PspOMI (1847) ApaI (1851) Eco32I - EcoRV (2090) BstHPI - HpaI - KspAI (2146) BsmBI - Esp3I (2255)