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Plasmid Files

pMCSG64 (linearized)

Linearized bacterial vector for ligation-independent cloning (LIC), with a 6xHis-FLAG®-TEV leader plus a second LIC site for expressing an untagged protein.

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pMCSG64 (linearized).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Midwest Center for Structural Genomics
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BssT1I - Eco130I - EcoT14I - ErhI - StyI (5148) Acc113I - BmcAI - ScaI - ZrmI (4396) BpvUI - Ple19I - PvuI (4286) PstI (4161) BsaI - Bso31I - BspTNI - Eco31I (3977) AhdI - AspEI - BmeRI - DriI - Eam1105I (3916) AlwNI - CaiI (3439) BspLU11I - PciI - PscI (3023) BspQI - LguI - PciSI - SapI (2907) BssNAI - Bst1107I - BstZ17I (2794) BsaAI - BstBAI - Ppu21I (2775) AspI - PflFI - PsyI - Tth111I (2768) BlpI - Bpu1102I - Bsp1720I - CelII (5171) PaeR7I - PspXI - Sfr274I - SlaI - XhoI (5249) BseX3I - BstZI - CciNI - EagI - EclXI - Eco52I - NotI (5257) HindIII (5264) SalI (5270) Ecl136II - Eco53kI - EcoICRI (5279) Psp124BI - SacI - SstI (5281) EcoRI (5283) BamHI (5289) Cfr9I - TspMI - XmaI - XmaCI (5323) SmaI (5325) End (5351) Start (0) Acc65I - Asp718I (25) KpnI (29) AanI - PsiI (49) BglII (56) FauNDI - NdeI (92) XbaI (130) BanIII * - Bsa29I * - BseCI * - BshVI - BspDI * - BspXI * - Bsu15I * - BsuTUI * - ClaI * (199) SgrAI (241) BbuI - PaeI - SphI (397) BstENI - EcoNI - XagI (457) AccB7I - PflMI - Van91I (504) BstAPI (605) MluI (922) BclI * - FbaI * - Ksp22I * (936) BstEII - BstPI - Eco91I - EcoO65I - PspEI (1103) Bsp120I - PspOMI (1129) ApaI (1133) BsePI - BssHII - PauI - PteI (1333) Eco32I - EcoRV (1372) BstHPI - HpaI - KspAI (1428) BoxI - BstPAI - PshAI (1767) FspAI (2004) PpuMI - Psp5II - PspPPI (2029) Bpu10I (2129)