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pNH-TrxT

Bacterial vector encoding an N-terminal 6xHis-thioredoxin-TEV cassette plus a SacB negative selection marker, for purification of recombinant proteins.

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pNH-TrxT.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Structural Genomics Consortium
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BglII (7536) SgrAI (7495) SphI (7347) BstAPI (7138) MluI (6814) BclI * (6800) BstEII (6632) NmeAIII (6614) ApaI (6611) PspOMI (6607) BssHII (6403) BglI (5757) FspI - FspAI (5736) PpuMI (5708) PflFI - Tth111I (4971) BspQI - SapI (4830) AlwNI (4304) NruI (3858) XbaI (0) NdeI (40) RsrII (171) BsaI (423) NcoI (424) ZraI (437) AatII (439) BfuAI - BspMI (457) StuI * (1128) MscI (1150) AanI - AanI (1527) BsrGI (1567) SnaBI (1636) SacII (1966) BsaI (2354) BamHI (2370) EcoRI (2376) Eco53kI (2384) SacI (2386) SalI (2389) HindIII (2395) EagI - NotI (2402) PaeR7I - PspXI - XhoI (2410) BlpI (2489) DraIII (2817) AanI - AanI (2942) AsiSI - PvuI (3517) TspMI - XmaI (3639) SmaI (3641) pNH-TrxT 7602 bp
BglII  (7536)
1 site
A G A T C T T C T A G A
SgrAI  (7495)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
SphI  (7347)
1 site
G C A T G C C G T A C G
BstAPI  (7138)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
MluI  (6814)
1 site
A C G C G T T G C G C A
BclI  (6800)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BstEII  (6632)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
NmeAIII  (6614)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
ApaI  (6611)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (6607)
1 site
G G G C C C C C