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pNIC-Zb

Bacterial vector encoding an N-terminal 6xHis-Z-basic-TEV cassette plus a SacB negative selection marker, for purification of recombinant proteins.

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pNIC-Zb.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Structural Genomics Consortium
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BglII (7427) SgrAI (7386) SphI (7238) BstAPI (7029) MluI (6705) BclI * (6691) BstEII (6523) NmeAIII (6505) ApaI (6502) PspOMI (6498) BssHII (6294) BglI (5648) FspAI (5627) PpuMI (5599) PflFI - Tth111I (4862) AlwNI (4195) NruI (3749) XbaI (47) NdeI (87) BsaI (314) NcoI (315) ZraI (328) AatII (330) StuI * (1019) MscI (1041) AanI - AanI (1418) BsrGI (1458) SnaBI (1527) SacII (1857) BsaI (2245) BamHI (2261) EcoRI (2267) SalI (2280) HindIII (2286) EagI - NotI (2293) PaeR7I - PspXI - XhoI (2301) BlpI (2380) DraIII (2708) AanI - AanI (2833) AsiSI - PvuI (3408) TspMI - XmaI (3530) SmaI (3532) pNIC-Zb 7446 bp
BglII  (7427)
1 site
A G A T C T T C T A G A
SgrAI  (7386)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
SphI  (7238)
1 site
G C A T G C C G T A C G
BstAPI  (7029)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
MluI  (6705)
1 site
A C G C G T T G C G C A
BclI  (6691)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BstEII  (6523)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
NmeAIII  (6505)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
ApaI  (6502)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (6498)
1 site
G G G C C C C C C G G G
BssHII  (6294)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
BglI  (5648)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
FspAI  (5627)
1 site
R T G C G C A Y Y A C G C G T R
PpuMI  (5599)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
PflFI  (4862)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4862)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
AlwNI  (4195)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
NruI  (3749)
1 site
T C G C G A A G C G C T
XbaI  (47)
1 site
T