Resources
Plasmid Files

T-Vector pMD19 (Simple)

Linearized vector with no MCS and with a single 3'-T at each end, for TA cloning of PCR products and blue-white screening.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

T-Vector pMD19 (Simple) Sequence and MapT-Vector pMD19 (Simple).dna
Map and Sequence File   
Sequence Author:  TaKaRa
Download Free Trial Get SnapGene Viewer

 EcoO109I (2251) AatII (2197) ZraI (2195) SspI (2079) XmnI (1874) ScaI (1755) TsoI (1674) NmeAIII (1423) BsrFI (1355) BpmI (1345) BsaI (1336) AhdI (1275) AlwNI (798) PfoI (2308) NdeI (2446) BstAPI (2447) KasI (2497) NarI (2498) SfoI (2499) PluTI (2501) End (2694) Start (1) BspQI - SapI (266) AflIII - PciI (382) BseYI (686) PspFI (690) T-Vector pMD19 (Simple) 2693 bp
EcoO109I  (2251)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
AatII  (2197)
1 site
G A C G T C C T G C A G
ZraI  (2195)
1 site
G A C G T C C T G C A G
SspI  (2079)
1 site
A A T A T T T T A T A A
XmnI  (1874)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (1755)
1 site
A G T A C T T C A T G A
TsoI  (1674)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
NmeAIII  (1423)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsrFI  (1355)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI  (1345)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BsaI  (1336)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (1275)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (798)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PfoI  (2308)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
NdeI  (2446)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
BstAPI  (2447)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
KasI  (2497)
1 site
G G C G C C C C G C G G
NarI  (2498)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
SfoI  (2499)
1 site
G G C G C C C C G C G G
PluTI  (2501)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
End  (2694)
0 sites
Start  (1)
0 sites
BspQI  (266)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (266)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
AflIII  (382)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (382)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BseYI  (686)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
PspFI  (690)
1 site
C C C A G C G G G T C G
AmpR
1202 .. 2062  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   1202 .. 1993  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1202 .. 2062  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   1994 .. 2062  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1202 .. 2062  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
443 .. 1031  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
443 .. 1031  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
lacZα
2408 .. 2693  =  286 bp
94 amino acids  =  10.8 kDa
Product: LacZα fragment of β-galactosidase
lacZα
2408 .. 2693  =  286 bp
94 amino acids  =  10.8 kDa
Product: LacZα fragment of β-galactosidase
AmpR promoter
2063 .. 2167  =  105 bp
AmpR promoter
2063 .. 2167  =  105 bp
lacZα
2 .. 45  =  44 bp
14 amino acids  =  1.5 kDa
Product: LacZα fragment of β-galactosidase
lacZα
2 .. 45  =  44 bp
14 amino acids  =  1.5 kDa
Product: LacZα fragment of β-galactosidase
lac promoter
89 .. 119  =  31 bp
   Segment 3:  -10  
   89 .. 95  =  7 bp
promoter for the E. coli lac operon
lac promoter
89 .. 119  =  31 bp
   Segment 2:  
   96 .. 113  =  18 bp
promoter for the E. coli lac operon
lac promoter
89 .. 119  =  31 bp
   Segment 1:  -35  
   114 .. 119  =  6 bp
promoter for the E. coli lac operon
lac promoter
89 .. 119  =  31 bp
3 segments
promoter for the E. coli lac operon
lac operator
65 .. 81  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
65 .. 81  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
M13 rev
41 .. 57  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
41 .. 57  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
2641 .. 2657  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
2641 .. 2657  =  17 bp
common sequencing primer, one of multiple similar
variants
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter