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Plasmid Files

pDrive (linearized)

Linearized bacterial vector with 3'-U overhangs for UA cloning and expression of a PCR product.

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pDrive (linearized).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Qiagen
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BspQI - SapI (3527) PciI (3410) PspFI (3110) BseYI (3106) AlwNI (3001) PflMI (2560) Bpu10I (2314) BsmBI (2313) AsiSI (2297) EcoNI (2209) SmaI (2171) TspMI - XmaI (2169) BspDI - ClaI (1988) NruI (1954) AhdI (1647) BsaI (1581) Acc65I (3802) KpnI (3806) SphI (3814) PstI (3819) MluI (3821) SnaBI (3829) BamHI (3835) EcoRI (3842) End (3853) Start (1) EcoRI (6) SalI (12) AccI (13) HincII (14) HindIII (18) PaeR7I - XhoI (24) AvrII - StyI (30) NheI (35) BmtI (39) XbaI (41) AleI - PmlI (52) BstXI (54) EcoO109I - PspOMI (59) ApaI (63) Eco53kI (67) SacI (69) EagI - NotI (72) AanI - PsiI (369) DraIII (497) BtgZI (498) NgoMIV (598) NaeI (600) BsaHI (1107) TatI (1164) ScaI (1166) NmeAIII (1500) BpmI (1578) pDrive 3852 bp
BspQI  (3527)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3527)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PciI  (3410)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
PspFI  (3110)
1 site
C C C A G C G G G T C G
BseYI  (3106)
1 site
C C