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Plasmid Files

pJAZZ®-OK GC (left arm)

Left arm of a linear bacterial vector with a kanamycin resistance gene, processed for GC cloning of DNA sequences that are unstable in circular plasmids.

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pJAZZ-OK GC (left arm) Sequence and MappJAZZ-OK GC (left arm).dna
Map and Sequence File   
Sequence Author:  Lucigen
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 10,000 7500 5000 2500 telL CB T7Te terminator N15 ori End (10,312) EagI - NotI (10,300) SpeI (10,293) SL1 (10,272 .. 10,292) XbaI (10,207) BssS α I (9891) RsrII (9656) PshAI (8685) BbvCI (8044) PluTI (7282) SfoI (7280) NarI (7279) KasI (7278) NdeI (7274) PaeR7I - PspXI - XhoI (6350) BsrGI (5170) AatII (4824) ZraI (4822) BstBI (4086) SexAI * (3996) PasI (3256) BssHII - MauBI (3125) SacI (1874) Eco53kI (1872) AarI (1785) PpuMI (1632) SgrDI (1402) StyI (1288) BstZ17I (1064) PmeI (956) MmeI (762) MfeI (248) Start (0) pJAZZ®-OK GC (left arm) 10,312 bp
End  (10,312)
0 sites
EagI  (10,300)
1 site
C G G C C G G C C G G C
NotI  (10,300)
1 site
G C G G C C G C C G C C G G C G
SpeI  (10,293)
1 site
A C T A G T T G A T C A
XbaI  (10,207)
1 site
T C T A G A A G A T C T
BssSαI  (9891)
1 site
C A C G A G G T G C T C
RsrII  (9656)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PshAI  (8685)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
BbvCI  (8044)
1 site
C C T C A G C G G A G T C G
PluTI  (7282)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (7280)
1 site
G G C G C C C C G C G G
NarI  (7279)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (7278)
1 site
G G C G C C C C G C G G
NdeI  (7274)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
PaeR7I  (6350)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (6350)
1 site
V C T C G A G B B G A G C T C V
XhoI  (6350)
1 site
C T C G A G G A G C T C
BsrGI  (5170)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
AatII  (4824)
1 site
G A C G T C C T G C A G
ZraI  (4822)
1 site
G A C G T C C T G C A G
BstBI  (4086)
1 site
T T C G A A A A G C T T
SexAI  (3996)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
PasI  (3256)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
BssHII  (3125)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
MauBI  (3125)
1 site
C G C G C G C G G C G C G C G C
SacI  (1874)
1 site
G A G C T C C T C G A G
Eco53kI  (1872)
1 site
G A G C T C C T C G A G
AarI  (1785)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI
recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its
electrophoretic mobility.
PpuMI  (1632)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
SgrDI  (1402)
1 site
C G T C G A C G G C A G C T G C
StyI  (1288)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BstZ17I  (1064)
1 site
G T A T A C C A T A T G
PmeI  (956)
1 site
G T T T A A A C C A A A T T T G
MmeI  (762)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI
recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
MfeI  (248)
1 site
C A A T T G G T T A A C
Start  (0)
0 sites
SL1
21-mer  /  57% GC
1 binding site
10,272 .. 10,292  =  21 annealed bases
Tm  =  59°C
sequencing primer
telL
1 .. 28  =  28 bp
telomeric sequence from coliphage N15
telL
1 .. 28  =  28 bp
telomeric sequence from coliphage N15
TelN
193 .. 2088  =  1896 bp
631 amino acids  =  72.3 kDa
Product: protelomerase of coliphage N15
generates the covalently closed hairpin telomeres
TelN
193 .. 2088  =  1896 bp
631 amino acids  =  72.3 kDa
Product: protelomerase of coliphage N15
generates the covalently closed hairpin telomeres
RepA
5084 .. 9058  =  3975 bp
1324 amino acids  =  149.5 kDa
Product: replication protein of coliphage N15
RepA
5084 .. 9058  =  3975 bp
1324 amino acids  =  149.5 kDa
Product: replication protein of coliphage N15
CB
9307 .. 9915  =  609 bp
202 amino acids  =  22.7 kDa
Product: repressor protein of coliphage N15
CB
9307 .. 9915  =  609 bp
202 amino acids  =  22.7 kDa
Product: repressor protein of coliphage N15
T7Te terminator
10,213 .. 10,240  =  28 bp
phage T7 early transcription terminator
T7Te terminator
10,213 .. 10,240  =  28 bp
phage T7 early transcription terminator
N15 ori
5335 .. 5424  =  90 bp
bidirectional replication origin of coliphage N15
N15 ori
5335 .. 5424  =  90 bp
bidirectional replication origin of coliphage N15
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