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Plasmid Files

pJAZZ®-OK GC (right arm)

Right arm of a linear bacterial vector with a kanamycin resistance gene, processed for GC cloning of DNA sequences that are unstable in circular plasmids.

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pJAZZ-OK GC (right arm) Sequence and MappJAZZ-OK GC (right arm).dna
Map and Sequence File   
Sequence Author:  Lucigen
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 2000 1500 1000 500 rrnB T1 terminator cat promoter KanR tonB terminator telR End (2207) PacI (1711) BssHII (1662) AccI (1526) NmeAIII (1476) BstEII (1432) PsiI (1364) PstI (1352) BsrDI (1331) BaeGI - Bme1580I (1317) BanI (1312) BtgI (1257) BsaI (1142) PvuII (1133) BglII (1101) PshAI (1060) AvrII - StyI (1049) PflMI (927) TaqII (918) AsiSI - PvuI (664) SspI (589) EcoNI (576) PasI (536) BspDI - ClaI (355) NruI (321) BanII (319) BmeT110I (263) AvaI - BsoBI - PaeR7I - PspXI - SmlI - XhoI (262) BsaAI (140) BmrI (120) NZ RevC (42 .. 63) SwaI (63) EagI - NotI (10) Start (1) pJAZZ®-OK GC (right arm) 2206 bp
End  (2207)
0 sites
PacI  (1711)
1 site
T T A A T T A A A A T T A A T T
BssHII  (1662)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
AccI  (1526)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
NmeAIII  (1476)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BstEII  (1432)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
PsiI  (1364)
1 site
T T A T A A A A T A T T
PstI  (1352)
1 site
C T G C A G G A C G T C
BsrDI  (1331)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
BaeGI  (1317)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (1317)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BanI  (1312)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
BtgI  (1257)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
BsaI  (1142)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PvuII  (1133)
1 site
C A G C T G G T C G A C
BglII  (1101)
1 site
A G A T C T T C T A G A
PshAI  (1060)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
AvrII  (1049)
1 site
C C T A G G G G A T C C
StyI  (1049)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
PflMI  (927)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
TaqII  (918)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
AsiSI  (664)
1 site
G C G A T C G C C G C T A G C G
PvuI  (664)
1 site
C G A T C G G C T A G C
SspI  (589)
1 site
A A T A T T T T A T A A
EcoNI  (576)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
PasI  (536)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
BspDI  (355)
1 site
A T C G A T T A G C T A
ClaI  (355)
1 site
A T C G A T T A G C T A
NruI  (321)
1 site
T C G C G A A G C G C T
BanII  (319)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BmeT110I  (263)
1 site
C Y C G R G G R G C Y C
AvaI  (262)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (262)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
PaeR7I  (262)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (262)
1 site
V C T C G A G B B G A G C T C V
SmlI  (262)
1 site
C T Y R A G G A R Y T C

Efficient cleavage requires at least two copies of the SmlI
recognition sequence.
Sticky ends from different SmlI sites may not be compatible.
XhoI  (262)
1 site
C T C G A G G A G C T C
BsaAI  (140)
1 site
Y A C G T R R T G C A Y
BmrI  (120)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
SwaI  (63)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
EagI  (10)
1 site
C G G C C G G C C G G C
NotI  (10)
1 site
G C G G C C G C C G C C G G C G
Start  (1)
0 sites
NZ RevC
22-mer  /  32% GC
1 binding site
42 .. 63  =  22 annealed bases
Tm  =  52°C
sequencing primer
rrnB T1 terminator
82 .. 127  =  46 bp
transcription terminator T1 from the E. coli rrnB
gene
rrnB T1 terminator
82 .. 127  =  46 bp
transcription terminator T1 from the E. coli rrnB
gene
cat promoter
131 .. 232  =  102 bp
promoter of the E. coli cat gene
cat promoter
131 .. 232  =  102 bp
promoter of the E. coli cat gene
KanR
233 .. 1048  =  816 bp
271 amino acids  =  30.9 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
KanR
233 .. 1048  =  816 bp
271 amino acids  =  30.9 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
tonB terminator
1062 .. 1093  =  32 bp
bidirectional E. coli tonB-P14 transcription
terminator
tonB terminator
1062 .. 1093  =  32 bp
bidirectional E. coli tonB-P14 transcription
terminator
telR
2180 .. 2207  =  28 bp
telomeric sequence from coliphage N15
telR
2180 .. 2207  =  28 bp
telomeric sequence from coliphage N15
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