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Plasmid Files

pLUG®-Multi (linearized)

Linearized TA cloning vector with 3'-T overhangs and a symmetric multiple cloning site for cloning of PCR products.

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pLUG-Multi (linearized).dna
Map and Sequence File:    Download    Open   
Sequence Author:  iNtRON Biotechnology
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BspQI - SapI (2463) AflIII - PciI (2346) PspFI (2046) BseYI (2042) AlwNI (1937) AhdI (1458) BsaI (1392) BpmI (1389) BsrFI (1373) NmeAIII (1311) TsoI (1060) EcoRI (2702) SacI (2712) KpnI (2718) SmaI (2720) BamHI (2723) XbaI (2729) SalI (2735) PstI (2745) SphI (2751) HindIII (2753) End (2766) Start (1) BmgBI (4) HindIII (10) SphI (20) PstI (26) SalI (28) XbaI (34) BamHI (40) SmaI (47) KpnI (53) SacI (59) EcoRI (61) KasI (231) NarI (232) SfoI (233) BbeI (235) NdeI (284) BstAPI (288) PfoI (419) EcoO109I (478) ZraI (537) AatII (539) SspI (653) XmnI (858) ScaI (977) pLUG®-Multi 2765 bp
BspQI  (2463)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2463)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AflIII  (2346)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (2346)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
PspFI  (2046)
1 site
C C C A G C G G G T C G
BseYI  (2042)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
AlwNI  (1937)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AhdI  (1458)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BsaI  (1392)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BpmI  (1389)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BsrFI  (1373)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
NmeAIII  (1311)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
TsoI  (1060)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
EcoRI  (2702)
2 sites
G A A T T C C T T A A G
SacI  (2712)
2 sites
G A G C T C C T C G A G
KpnI  (2718)
2 sites
G G T A C C C C A T G G
SmaI  (2720)
2 sites
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (2723)
2 sites
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (2729)
2 sites
T C T A G A A G A T C T
SalI  (2735)
2 sites
G T C G A C C A G C T G
PstI  (2745)
2 sites
C T G C A G G A C G T C
SphI  (2751)
2 sites
G C A T G C C G T A C G
HindIII  (2753)
2 sites
A A G C T T T T C G A A
End  (2766)
0 sites
Start  (1)
0 sites
BmgBI  (4)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
HindIII  (10)
2 sites
A A G C T T T T C G A A
SphI  (20)
2 sites
G C A T G C C G T A C G
PstI  (26)
2 sites
C T G C A G G A C G T C
SalI  (28)
2 sites
G T C G A C C A G C T G
XbaI  (34)
2 sites
T C T A G A A G A T C T
BamHI  (40)
2 sites
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SmaI  (47)
2 sites
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
KpnI  (53)
2 sites
G G T A C C C C A T G G
SacI  (59)
2 sites
G A G C T C C T C G A G
EcoRI  (61)
2 sites
G A A T T C C T T A A G
KasI  (231)
1 site
G G C G C C C C G C G G
NarI  (232)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (233)
1 site
G G C G C C C C G C G G
BbeI  (235)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the BbeI recognition sequence.
NdeI  (284)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BstAPI  (288)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
PfoI  (419)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
EcoO109I  (478)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
ZraI  (537)
1 site
G A C G T C C T G C A G
AatII  (539)
1 site
G A C G T C C T G C A G
SspI  (653)
1 site
A A T A T T T T A T A A
XmnI  (858)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (977)
1 site
A G T A C T T C A T G A
AmpR
671 .. 1531  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   671 .. 739  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
671 .. 1531  =  861 bp
286 amino acids  =  31.6 kDa
&