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pLUG® (linearized)

Linearized TA cloning vector with 3'-T overhangs for cloning of PCR products.

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pLUG (linearized).dna
Map and Sequence File:    Download    Open   
Sequence Author:  iNtRON Biotechnology
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BspQI - SapI (2622) NspI (2509) AflIII - PciI (2505) PspFI (2205) BseYI (2201) AlwNI (2096) AhdI (1617) BsaI (1551) NmeAIII (1470) ScaI (1136) TatI (1134) BsaHI (1077) Eco53kI (2905) SacI (2907) BtgI (2911) AleI (2913) SacII (2914) BstXI (2915) EagI - NotI (2920) XbaI (2927) SpeI (2933) BamHI (2939) AvaI - BsoBI - TspMI - XmaI (2945) BmeT110I (2946) SmaI (2947) PstI (2955) EcoRI (2957) EcoRV (2965) HindIII (2969) End (2982) Start (1) BmgBI (4) HindIII (10) BspDI - ClaI (17) SalI (25) AccI (26) HincII (27) EcoO109I - PspOMI (40) ApaI (44) Acc65I (46) KpnI (50) PsiI (339) BsaAI - DraIII (467) BtgZI (468) NgoMIV (568) NaeI (570) XmnI (1017) pLUG® 2981 bp
BspQI  (2622)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2622)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
NspI  (2509)
1 site
R C A T G Y Y G T A C R
AflIII  (2505)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (2505)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
PspFI  (2205)
1 site
C C C A G C G G G T C G
BseYI  (2201)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
AlwNI  (2096)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AhdI  (1617)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BsaI  (1551)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
NmeAIII  (1470)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
ScaI  (1136)
1 site
A G T A C T T C A T G A
TatI  (1134)
1 site
W G T A C W W C A T G W
BsaHI  (1077)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
Eco53kI  (2905)
1 site
G A G C T C C T C G A G
SacI  (2907)
1 site
G A G C T C C T C G A G
BtgI  (2911)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
AleI  (2913)
1 site
C A C N N N N G T G G T G N N N N C A C
SacII  (2914)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
BstXI  (2915)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
EagI  (2920)
1 site
C G G C C G G C C G G C
NotI  (2920)
1 site