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pPrime (linearized)

Linearized vector with 3'-U overhangs for UA cloning of PCR products produced by non-proofreading DNA polymerases.

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pPrime (linearized).dna
Map and Sequence File:    Download    Open   
Sequence Author:  5 Prime
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BspQI - SapI (3527) PciI (3410) PspFI (3110) BseYI (3106) AlwNI (3001) PflMI (2560) Bpu10I (2314) BsmBI (2313) AsiSI (2297) EcoNI (2209) SmaI (2171) TspMI - XmaI (2169) BspDI - ClaI (1988) NruI (1954) AhdI (1647) BsaI (1581) Acc65I (3802) KpnI (3806) SphI (3814) PstI (3819) MluI (3821) SnaBI (3829) BamHI (3835) EcoRI (3842) End (3853) Start (1) EcoRI (6) SalI (12) AccI (13) HincII (14) HindIII (18) PaeR7I - XhoI (24) AvrII - StyI (30) NheI (35) BmtI (39) XbaI (41) AleI - PmlI (52) BstXI (54) EcoO109I - PspOMI (59) ApaI (63) Eco53kI (67) SacI (69) EagI - NotI (72) AanI - PsiI (369) DraIII (497) BtgZI (498) NgoMIV (598) NaeI (600) BsaHI (1107) TatI (1164) ScaI (1166) NmeAIII (1500) BpmI (1578) pPrime 3852 bp
BspQI  (3527)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3527)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PciI  (3410)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
PspFI  (3110)
1 site
C C C A G C G G G T C G
BseYI  (3106)
1 site