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pSC-A-amp/kan (linearized)

Linearized vector with modified 3'-U overhangs and bound topoisomerase, for efficient TA cloning of PCR products.

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pSC-A-amp kan (linearized).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Agilent Technologies
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Acc65I (4204) BspQI - SapI (3923) AflIII - PciI (3806) AlwNI (3397) BclI * (3046) Bsu36I (2982) AgeI (2932) BstBI (2858) DraIII (2408) SspI (2017) KpnI (4208) PspOMI (4210) EcoO109I (4211) ApaI (4214) AbsI - PaeR7I - PspXI - XhoI (4219) SalI (4225) AccI (4226) HincII (4227) BspDI - ClaI (4235) HindIII (4240) EcoRV (4248) EcoRI (4259) End (4270) Start (1) EcoRI (7) PstI (26) TspMI - XmaI (28) SmaI (30) BamHI (34) SpeI (40) XbaI (46) NotI (53) SacII (64) Eco53kI (69) SacI (71) RsrII (483) NcoI - StyI (566) SphI (601) MscI (920) KasI (999) NarI (1000) SfoI (1001) PluTI (1003) AhdI (1213) BsaI (1274) BpmI (1283) PvuI (1583) XmnI (1812) pSC-A-amp/kan 4269 bp
Acc65I  (4204)
1 site
G G T A C C C C A T G G
BspQI  (3923)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3923)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AflIII  (3806)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (3806)
1 site
A C A