Resources
Plasmid Files

pTrcHis-TOPO® (linearized)

Linearized bacterial vector with 3'-T overhangs and bound topoisomerase, for TOPO® TA cloning and inducible expression of N-terminally 6xHis-tagged proteins.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pTrcHis-TOPO (linearized).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
Download Free Trial Get SnapGene Viewer


PluTI (3745) SfoI (3743) NarI * (3742) KasI (3741) HpaI (3608) EcoRV (3552) ApaI - BanII (3313) PspOMI (3309) BstEII (3283) BclI * (3116) MluI (3102) PflMI (2684) NsiI (2665) SphI (2663) MauBI (2641) PfoI (2573) PflFI - Tth111I (2474) BsaAI (2468) BstZ17I (2449) AccI (2448) NdeI (2397) BspQI - SapI (2335) PciI (2218) AlwNI (1809) BfuAI - BspMI (3945) BtgI - NcoI - StyI (4284) NheI (4322) BmtI (4326) BsaBI * (4377) BamHI (4378) End (4390) Start (1) EcoRI (7) BstBI (16) HindIII (19) ScaI (849) PvuI (961) FspI (1107) BglI (1212) AhdI (1330) pTrcHis-TOPO® 4389 bp
PluTI  (3745)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (3743)
1 site
G G C G C C C C G C G G
NarI  (3742)
1 site
G G C G C C C C G C G G
* Blocked by Dcm methylation.
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (3741)
1 site
G G C G C C C C G C G G
HpaI  (3608)
1 site
G T T A A C C A A T T G
EcoRV  (3552)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
ApaI  (3313)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BanII  (3313)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
PspOMI  (3309)
1 site
G G G C C C C C