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Plasmid Files

pcDNA™4/HisMax-TOPO® (linearized)

Linearized mammalian vector with 3'-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products and expression of N-terminally 6xHis-tagged proteins.

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pcDNA4 HisMax-TOPO (linearized).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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SgrDI (4088) SspI (3971) ScaI (3647) PvuI (3537) FspI (3389) AhdI (3167) PciI (2277) BspQI - SapI (2161) BstZ17I (1898) BsmI (1846) FseI (1538) BmgBI (1396) SgrAI (1376) BglII (4102) MfeI (4251) Bpu10I (4270) NruI (4298) MluI (4318) SpeI (4339) NdeI (4574) SnaBI (4680) Eco53kI (4906) SacI (4908) AflII (4998) HindIII (5001) BlpI (5004) SrfI (5051) End (5274) Start (1) BamHI (16) EcoRI (31) PstI (40) EcoRV (43) NotI (58) PaeR7I - PspXI - XhoI (64) XbaI (70) PspOMI (76) ApaI (80) BbsI (296) StuI (1132) MscI (1265) MauBI (1298) pcDNA™4/HisMax-TOPO® 5273 bp