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Plasmid Files

pcDNA™6.2/C-EmGFP-GW TOPO® (linearized)

Linearized vector with 3'-T overhangs and bound topoisomerase, for TOPO® TA cloning to add a C-terminal GFP tag and create a Gateway® expression clone.

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pcDNA6.2 C-EmGFP-GW TOPO (linearized).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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SgrDI (4859) SspI (4742) Acc113I - BmcAI - ScaI - ZrmI (4418) AhdI - AspEI - BmeRI - DriI - Eam1105I (3938) BspLU11I - PciI - PscI (3045) BspQI - LguI - PciSI - SapI (2929) BssNAI - Bst1107I - BstZ17I (2666) BsaMI - BsmI - Mva1269I - PctI (2614) BsaBI * - Bse8I - BseJI * - MamI * (2276) PpuMI - Psp5II - PspPPI (2137) BseX3I - BstZI - EagI - EclXI - Eco52I (2094) BbsI - BpiI - BpuAI - BstV2I (2063) BalI - MlsI - MluNI - MscI - Msp20I (1981) BclI * - FbaI * - Ksp22I * (1900) SmaI (1872) BmeT110I (1871) Ama87I - AvaI - BsiHKCI - BsoBI - Cfr9I - Eco88I - TspMI - XmaI - XmaCI (1870) MfeI - MunI (5022) MluI (5089) AhlI - BcuI - SpeI (5110) FauNDI - NdeI (5345) BstSNI - Eco105I - SnaBI (5451) Ecl136II - Eco53kI - EcoICRI (5677) Psp124BI - SacI - SstI (5679) End (5814) Start (1) BstHPI - HpaI - KspAI (48) MssI - PmeI (781) CsiI - MabI - SexAI * (1616) AatI - Eco147I - PceI - SseBI - StuI (1848) AspA2I - AvrII - BlnI - XmaJI (1849)