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Plasmid Files

pcDNA™6.2/N-EmGFP-GW TOPO® (linearized)

Linearized vector with 3'-T overhangs and bound topoisomerase, for TOPO® TA cloning to add an N-terminal GFP tag and create a Gateway® expression clone.

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pcDNA6.2 N-EmGFP-GW TOPO (linearized).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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SgrDI (4187) SspI (4070) Acc113I - BmcAI - ScaI - ZrmI (3746) AhdI - AspEI - BmeRI - DriI - Eam1105I (3266) BspLU11I - PciI - PscI (2373) BspQI - LguI - PciSI - SapI (2257) BssNAI - Bst1107I - BstZ17I (1994) BsaMI - BsmI - Mva1269I - PctI (1942) BlpI - Bpu1102I - Bsp1720I - CelII (1701) BsaBI * - Bse8I - BseJI * - MamI * (1604) PpuMI - Psp5II - PspPPI (1465) BseX3I - BstZI - EagI - EclXI - Eco52I (1422) BalI - MlsI - MluNI - MscI - Msp20I (1309) BclI * - FbaI * - Ksp22I * (1228) SmaI (1200) Cfr9I - TspMI - XmaI - XmaCI (1198) AspA2I - AvrII - BlnI - XmaJI (1177) MfeI - MunI (4350) AhlI - BcuI - SpeI (4438) FauNDI - NdeI (4673) BstSNI - Eco105I - SnaBI (4779) Ecl136II - Eco53kI - EcoICRI (5005) Psp124BI - SacI - SstI (5007) BsmBI - Esp3I (5028) AccIII - Aor13HI - BseAI - Bsp13I - BspEI - Kpn2I - MroI (5090) XbaI (5097) BamHI (5103) Eco32I - EcoRV (5119) PspXI (5851) End (5902) Start (1) MssI - PmeI (109) CsiI - MabI - SexAI * (944) AatI - Eco147I - PceI - SseBI - StuI (1176)