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Plasmid Files

pAdenoX-PRLS-DsRed-Express Linear

In-Fusion®-ready linearized promoterless adenoviral vector for inserting a gene cassette and co-expressing with DsRed-Express.

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pAdenoX-PRLS-DsRed-Express Linear Sequence and MappAdenoX-PRLS-DsRed-Express Linear.dna
Map and Sequence File   
Sequence Author:  Clontech
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 End (34,313) In-Fusion Cloning site Adeno-X fwd screening primer (34,051 .. 34,075) M13 rev AmpR promoter BstBI (30,980) SV40 poly(A) signal CMV promoter I-PpoI (24,385) SrfI (24,036) SpeI (23,588) MreI (22,901) BamHI (18,074) Start (0) Adeno-X rev screening primer (19 .. 38) BstZ17I (2280) PmeI (9770) AsiSI (17,954) pAdenoX-PRLS-DsRed-Express Linear 34,313 bp
End  (34,313)
0 sites
BstBI  (30,980)
1 site
T T C G A A A A G C T T
I-PpoI  (24,385)
1 site
C T C T C T T A A G G T A G C G A G A G A A T T C C A T C G

I-PpoI is a homing endonuclease that can recognize a variety of
similar recognition sequences.
SrfI  (24,036)
1 site
G C C C G G G C C G G G C C C G
SpeI  (23,588)
1 site
A C T A G T T G A T C A
MreI  (22,901)
1 site
C G C C G G C G G C G G C C G C
BamHI  (18,074)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
Start  (0)
0 sites
BstZ17I  (2280)
1 site
G T A T A C C A T A T G
PmeI  (9770)
1 site
G T T T A A A C C A A A T T T G
AsiSI  (17,954)
1 site
G C G A T C G C C G C T A G C G
Adeno-X fwd screening primer
25-mer  /  52% GC
1 binding site
34,051 .. 34,075  =  25 annealed bases
Tm  =  63°C
Adeno-X rev screening primer
20-mer  /  50% GC
1 binding site
19 .. 38  =  20 annealed bases
Tm  =  57°C
adenovirus DNA
39 .. 24,369  =  24,331 bp
adenovirus DNA
39 .. 24,369  =  24,331 bp
adenovirus DNA
26,020 .. 30,855  =  4836 bp
adenovirus DNA
26,020 .. 30,855  =  4836 bp
AmpR
31,859 .. 32,719  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   31,859 .. 31,927  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
31,859 .. 32,719  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   31,928 .. 32,719  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
31,859 .. 32,719  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
DsRed-Express
25,063 .. 25,740  =  678 bp
225 amino acids  =  25.7 kDa
DsRed-Express
25,063 .. 25,740  =  678 bp
225 amino acids  =  25.7 kDa
ori
32,890 .. 33,478  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
32,890 .. 33,478  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
31,220 .. 31,675  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
31,220 .. 31,675  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
CMV enhancer
24,496 .. 24,799  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
24,496 .. 24,799  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
24,800 .. 25,003  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
24,800 .. 25,003  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
Ad5 Ψ
34,138 .. 34,288  =  151 bp
packaging signal for adenovirus serotype 5
Ad5 Ψ
34,138 .. 34,288  =  151 bp
packaging signal for adenovirus serotype 5
AmpR promoter
31,754 .. 31,858  =  105 bp
AmpR promoter
31,754 .. 31,858  =  105 bp
ITR
30,856 .. 30,958  =  103 bp
inverted terminal repeat of human adenovirus
serotype 5
ITR
30,856 .. 30,958  =  103 bp
inverted terminal repeat of human adenovirus
serotype 5
ITR
33,948 .. 34,050  =  103 bp
inverted terminal repeat of human adenovirus
serotype 5
ITR
33,948 .. 34,050  =  103 bp
inverted terminal repeat of human adenovirus
serotype 5
SV40 poly(A) signal
25,861 .. 25,942  =  82 bp
SV40 polyadenylation signal
SV40 poly(A) signal
25,861 .. 25,942  =  82 bp
SV40 polyadenylation signal
M13 fwd
31,063 .. 31,079  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
31,063 .. 31,079  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
33,864 .. 33,880  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
33,864 .. 33,880  =  17 bp
common sequencing primer, one of multiple similar
variants
In-Fusion Cloning site
1 .. 15  =  15 bp
sequence to be added to 5' end of reverse primer
In-Fusion Cloning site
1 .. 15  =  15 bp
sequence to be added to 5' end of reverse primer
In-Fusion Cloning site
34,299 .. 34,313  =  15 bp
sequence to be added to 5' end of forward primer
In-Fusion Cloning site
34,299 .. 34,313  =  15 bp
sequence to be added to 5' end of forward primer
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