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Plasmid Files

pAdenoX-PRLS Linear

In-Fusion®-ready linearized promoterless adenoviral vector for inserting an entire gene expression cassette.

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pAdenoX-PRLS Linear Sequence and MappAdenoX-PRLS Linear.dna
Map and Sequence File   
Sequence Author:  Clontech
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 End (32,665) In-Fusion Cloning site Adeno-X fwd screening primer (32,403 .. 32,427) ITR M13 rev M13 fwd XbaI (29,360) BstBI (29,332) SrfI (24,036) SpeI (23,588) MreI (22,901) BamHI (18,074) Start (0) Adeno-X rev screening primer (19 .. 38) BstZ17I (2280) PmeI (9770) AsiSI (17,954) pAdenoX-PRLS Linear 32,665 bp
End  (32,665)
0 sites
XbaI  (29,360)
1 site
T C T A G A A G A T C T
BstBI  (29,332)
1 site
T T C G A A A A G C T T
SrfI  (24,036)
1 site
G C C C G G G C C G G G C C C G
SpeI  (23,588)
1 site
A C T A G T T G A T C A
MreI  (22,901)
1 site
C G C C G G C G G C G G C C G C
BamHI  (18,074)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
Start  (0)
0 sites
BstZ17I  (2280)
1 site
G T A T A C C A T A T G
PmeI  (9770)
1 site
G T T T A A A C C A A A T T T G
AsiSI  (17,954)
1 site
G C G A T C G C C G C T A G C G
Adeno-X fwd screening primer
25-mer  /  52% GC
1 binding site
32,403 .. 32,427  =  25 annealed bases
Tm  =  63°C
Adeno-X rev screening primer
20-mer  /  50% GC
1 binding site
19 .. 38  =  20 annealed bases
Tm  =  57°C
adenovirus DNA
39 .. 29,207  =  29,169 bp
adenovirus DNA
39 .. 29,207  =  29,169 bp
AmpR
30,211 .. 31,071  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   30,211 .. 30,279  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
30,211 .. 31,071  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   30,280 .. 31,071  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
30,211 .. 31,071  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
31,242 .. 31,830  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
31,242 .. 31,830  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
29,572 .. 30,027  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
29,572 .. 30,027  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
Ad5 Ψ
32,490 .. 32,640  =  151 bp
packaging signal for adenovirus serotype 5
Ad5 Ψ
32,490 .. 32,640  =  151 bp
packaging signal for adenovirus serotype 5
AmpR promoter
30,106 .. 30,210  =  105 bp
AmpR promoter
30,106 .. 30,210  =  105 bp
ITR
29,208 .. 29,310  =  103 bp
inverted terminal repeat of human adenovirus
serotype 5
ITR
29,208 .. 29,310  =  103 bp
inverted terminal repeat of human adenovirus
serotype 5
ITR
32,300 .. 32,402  =  103 bp
inverted terminal repeat of human adenovirus
serotype 5
ITR
32,300 .. 32,402  =  103 bp
inverted terminal repeat of human adenovirus
serotype 5
M13 fwd
29,415 .. 29,431  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
29,415 .. 29,431  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
32,216 .. 32,232  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
32,216 .. 32,232  =  17 bp
common sequencing primer, one of multiple similar
variants
In-Fusion Cloning site
1 .. 15  =  15 bp
sequence to be added to 5' end of reverse primer
In-Fusion Cloning site
1 .. 15  =  15 bp
sequence to be added to 5' end of reverse primer
In-Fusion Cloning site
32,651 .. 32,665  =  15 bp
sequence to be added to 5' end of forward primer
In-Fusion Cloning site
32,651 .. 32,665  =  15 bp
sequence to be added to 5' end of forward primer
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