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pAdenoX-Tet3G Linear

In-Fusion®-ready linearized adenoviral vector for doxycycline-inducible expression of a gene.

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pAdenoX-Tet3G Linear Sequence and MappAdenoX-Tet3G Linear.dna
Map and Sequence File   
Sequence Author:  Clontech
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 End (36,043) In-Fusion Cloning site Adeno-X fwd screening primer (35,368 .. 35,392) ITR M13 rev AmpR promoter BstBI (32,297) SV40 poly(A) signal CMV promoter I-PpoI (25,302) SrfI (24,953) MreI (23,818) AsiSI (18,871) Start (0) In-Fusion Cloning site stop codons small t intron Adeno-X rev screening primer (936 .. 955) BstZ17I (3197) PmeI (10,687) pAdenoX-Tet3G Linear 36,043 bp
End  (36,043)
0 sites
BstBI  (32,297)
1 site
T T C G A A A A G C T T
I-PpoI  (25,302)
1 site
C T C T C T T A A G G T A G C G A G A G A A T T C C A T C G

I-PpoI is a homing endonuclease that can recognize a variety of
similar recognition sequences.
SrfI  (24,953)
1 site
G C C C G G G C C G G G C C C G
MreI  (23,818)
1 site
C G C C G G C G G C G G C C G C
AsiSI  (18,871)
1 site
G C G A T C G C C G C T A G C G
Start  (0)
0 sites
BstZ17I  (3197)
1 site
G T A T A C C A T A T G
PmeI  (10,687)
1 site
G T T T A A A C C A A A T T T G
Adeno-X fwd screening primer
25-mer  /  52% GC
1 binding site
35,368 .. 35,392  =  25 annealed bases
Tm  =  63°C
Adeno-X rev screening primer
20-mer  /  50% GC
1 binding site
936 .. 955  =  20 annealed bases
Tm  =  57°C
adenovirus DNA
956 .. 25,286  =  24,331 bp
adenovirus DNA
956 .. 25,286  =  24,331 bp
adenovirus DNA
27,337 .. 32,172  =  4836 bp
adenovirus DNA
27,337 .. 32,172  =  4836 bp
AmpR
33,176 .. 34,036  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   33,176 .. 33,244  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
33,176 .. 34,036  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   33,245 .. 34,036  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
33,176 .. 34,036  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
Tet-On 3G
26,097 .. 26,843  =  747 bp
248 amino acids  =  27.7 kDa
Product: modified rtTA protein that binds tightly to
promoters containing the tet operator in the
presence of doxycycline
Tet-On 3G
26,097 .. 26,843  =  747 bp
248 amino acids  =  27.7 kDa
Product: modified rtTA protein that binds tightly to
promoters containing the tet operator in the
presence of doxycycline
ori
34,207 .. 34,795  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
34,207 .. 34,795  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
32,537 .. 32,992  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
32,537 .. 32,992  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
CMV enhancer
25,411 .. 25,790  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
25,411 .. 25,790  =  380 bp
human cytomegalovirus immediate early enhancer
TRE3G promoter
35,645 .. 36,020  =  376 bp
3rd-generation Tet-responsive promoter that can be
activated by binding of Tet-On 3G
TRE3G promoter
35,645 .. 36,020  =  376 bp
3rd-generation Tet-responsive promoter that can be
activated by binding of Tet-On 3G
CMV promoter
25,791 .. 25,994  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
25,791 .. 25,994  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
Ad5 Ψ
35,455 .. 35,605  =  151 bp
packaging signal for adenovirus serotype 5
Ad5 Ψ
35,455 .. 35,605  =  151 bp
packaging signal for adenovirus serotype 5
SV40 poly(A) signal
26,853 .. 26,987  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
26,853 .. 26,987  =  135 bp
SV40 polyadenylation signal
AmpR promoter
33,071 .. 33,175  =  105 bp
AmpR promoter
33,071 .. 33,175  =  105 bp
ITR
32,173 .. 32,275  =  103 bp
inverted terminal repeat of human adenovirus
serotype 5
ITR
32,173 .. 32,275  =  103 bp
inverted terminal repeat of human adenovirus
serotype 5
ITR
35,265 .. 35,367  =  103 bp
inverted terminal repeat of human adenovirus
serotype 5
ITR
35,265 .. 35,367  =  103 bp
inverted terminal repeat of human adenovirus
serotype 5
SV40 poly(A) signal
822 .. 903  =  82 bp
SV40 polyadenylation signal
SV40 poly(A) signal
822 .. 903  =  82 bp
SV40 polyadenylation signal
small t intron
182 .. 247  =  66 bp
simian virus 40 (SV40) small t antigen intron
small t intron
182 .. 247  =  66 bp
simian virus 40 (SV40) small t antigen intron
M13 fwd
32,380 .. 32,396  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
32,380 .. 32,396  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
35,181 .. 35,197  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
35,181 .. 35,197  =  17 bp
common sequencing primer, one of multiple similar
variants
In-Fusion Cloning site
1 .. 15  =  15 bp
sequence to be added to 5' end of reverse primer
In-Fusion Cloning site
1 .. 15  =  15 bp
sequence to be added to 5' end of reverse primer
In-Fusion Cloning site
36,029 .. 36,043  =  15 bp
sequence to be added to 5' end of forward primer
In-Fusion Cloning site
36,029 .. 36,043  =  15 bp
sequence to be added to 5' end of forward primer
stop codons
35 .. 45  =  11 bp
stop codons in all three reading frames
stop codons
35 .. 45  =  11 bp
stop codons in all three reading frames
tet operator
35,650 .. 35,668  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,650 .. 35,668  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,686 .. 35,704  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,686 .. 35,704  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,722 .. 35,740  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,722 .. 35,740  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,758 .. 35,776  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,758 .. 35,776  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,794 .. 35,812  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,794 .. 35,812  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,830 .. 35,848  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,830 .. 35,848  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,866 .. 35,884  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,866 .. 35,884  =  19 bp
bacterial operator O2 for the tetR and tetA genes
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