pLMN-ZsGreen-Neomycin

Retroviral vector with a ZsGreen marker, for expressing a short hairpin RNA (shRNA) with an "UltramiR" microRNA scaffold.

Sequence Author: transOMIC

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No matches
pLMN-ZsGreen-Neomycin 8065 bp
AmpR
6271 .. 7131  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   6271 .. 7062  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6271 .. 7131  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   7063 .. 7131  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6271 .. 7131  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
NeoR/KanR
2270 .. 3073  =  804 bp
267 amino acids  =  29.3 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
2270 .. 3073  =  804 bp
267 amino acids  =  29.3 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
ZsGreen1
3659 .. 4354  =  696 bp
231 amino acids  =  26.1 kDa
Product: Zoanthus green fluorescent protein
mammalian codon-optimized
ZsGreen1
3659 .. 4354  =  696 bp
231 amino acids  =  26.1 kDa
Product: Zoanthus green fluorescent protein
mammalian codon-optimized
ori
5512 .. 6100  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
5512 .. 6100  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
IRES
3096 .. 3647  =  552 bp
internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)
IRES
3096 .. 3647  =  552 bp
internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)
5' LTR
8064 .. 515  =  517 bp
5' long terminal repeat from murine embryonic stem cell virus
5' LTR
8064 .. 515  =  517 bp
5' long terminal repeat from murine embryonic stem cell virus
3' LTR
4458 .. 4972  =  515 bp
3' long terminal repeat from murine embryonic stem cell virus
3' LTR
4458 .. 4972  =  515 bp
3' long terminal repeat from murine embryonic stem cell virus
PGK promoter
1757 .. 2256  =  500 bp
mouse phosphoglycerate kinase 1 promoter
PGK promoter
1757 .. 2256  =  500 bp
mouse phosphoglycerate kinase 1 promoter
gag (truncated)
987 .. 1403  =  417 bp
truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon
gag (truncated)
987 .. 1403  =  417 bp
truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon
MESV Ψ
579 .. 920  =  342 bp
packaging signal of murine embryonic stem cell virus
MESV Ψ
579 .. 920  =  342 bp
packaging signal of murine embryonic stem cell virus
3' miR-30a
1610 .. 1736  =  127 bp
sequence downstream of the 71-nt precursor of the human miR-30a microRNA (Zeng et al., 2002)
3' miR-30a
1610 .. 1736  =  127 bp
sequence downstream of the 71-nt precursor of the human miR-30a microRNA (Zeng et al., 2002)
5' miR-30a
1419 .. 1540  =  122 bp
sequence upstream of the 71-nt precursor of the human miR-30a microRNA (Zeng et al., 2002)
5' miR-30a
1419 .. 1540  =  122 bp
sequence upstream of the 71-nt precursor of the human miR-30a microRNA (Zeng et al., 2002)
AmpR promoter
7132 .. 7236  =  105 bp
AmpR promoter
7132 .. 7236  =  105 bp
shRNA
1541 .. 1609  =  69 bp
3 segments
   Segment 1:  
   1541 .. 1567  =  27 bp
short hairpin RNA
shRNA
1541 .. 1609  =  69 bp
3 segments
   Segment 2:  miR-30a loop  
   1568 .. 1582  =  15 bp
short hairpin RNA
shRNA
1541 .. 1609  =  69 bp
3 segments
   Segment 3:  
   1583 .. 1609  =  27 bp
short hairpin RNA
shRNA
1541 .. 1609  =  69 bp
3 segments
short hairpin RNA
ORF:  2270 .. 3073  =  804 bp
ORF:  267 amino acids  =  29.3 kDa
ORF:  3647 .. 4354  =  708 bp
ORF:  235 amino acids  =  26.5 kDa
ORF:  6401 .. 6667  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  7517 .. 7813  =  297 bp
ORF:  98 amino acids  =  11.7 kDa
ORF:  2037 .. 2450  =  414 bp
ORF:  137 amino acids  =  15.1 kDa
ORF:  2451 .. 2837  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  362 .. 637  =  276 bp
ORF:  91 amino acids  =  9.9 kDa
ORF:  674 .. 934  =  261 bp
ORF:  86 amino acids  =  9.5 kDa
ORF:  2588 .. 2842  =  255 bp
ORF:  84 amino acids  =  9.6 kDa
ORF:  4036 .. 4329  =  294 bp
ORF:  97 amino acids  =  10.9 kDa
ORF:  6271 .. 7131  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  7581 .. 7811  =  231 bp
ORF:  76 amino acids  =  7.5 kDa
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Download pLMN-ZsGreen-Neomycin.dna file

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