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pLVX-EF1α-Tet3G

Lentiviral vector encoding the Tet-On 3G transactivator protein, which allows for inducible gene expression.

To see this sequence with restriction sites, features, and translations, please download
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pLVX-EF1alpha-Tet3G.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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SgrDI (9712) SspI (9595) BpvUI - Ple19I - PvuI (9161) AhdI - AspEI - BmeRI - DriI - Eam1105I (8791) BmtI - BspOI (7517) AsuNHI - NheI (7513) PfoI (6275) MluI (5989) AfeI - Aor51HI - Eco47III (5928) CpoI - CspI - RsrII - Rsr2I (5655) AspI - PflFI - PsyI - Tth111I (5257) BalI - MlsI - MluNI - MscI - Msp20I (5221) KflI - PpuMI - Psp5II - PspPPI - SanDI (1934) Bsp1407I - BsrGI - BstAUI - SspBI (2229) AgeI - AsiGI - BshTI - CspAI - PinAI (2465) BsmBI - Esp3I (3595) FauNDI - NdeI (4249) Cfr9I - TspMI - XmaI - XmaCI (4408) SmaI (4410) BamHI (4417) AspA2I - AvrII - BlnI - XmaJI (4576) AarI (4764) AjiI - BmgBI - BtrI (4967) pLVX-EF1 α- Tet3G 10,080 bp
SgrDI  (9712)
1 site
C G T C G A C G G C A G C T G C
SspI  (9595)
1 site
A A T A T T T T A T A A
BpvUI  (9161)
1 site
C G A T C G G C T A G C
Ple19I  (9161)
1 site
C G A T C G G C T A G C
PvuI  (9161)
1 site
C G A T C G G C T A G C
AhdI  (8791)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AspEI  (8791)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AspEI may be hard to ligate.
Sticky ends from different AspEI sites may not be compatible.
BmeRI  (8791)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by BmeRI may be hard to ligate.
Sticky ends from different BmeRI sites may not be compatible.
DriI  (8791)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by DriI may be hard to ligate.
Sticky ends from different DriI sites may not be compatible.
Eam1105I  (8791)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by Eam1105I may be hard to ligate.
Sticky ends from different Eam1105I sites may not be compatible.
BmtI  (7517)
1 site
G C T A G C C G A T C G
BspOI  (7517)
1 site
G C T A G C C G A T C G
AsuNHI  (7513)
1 site
G C T A G C C G A T C G
NheI  (7513)
1 site
G C T A G C C G A T C G
PfoI  (6275)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
MluI  (5989)
1 site
A C G C G T T G C G C A
AfeI  (5928)
1 site
A G C G C T T C G C G A
Aor51HI  (5928)
1 site
A G C G C T T C G C G A
Eco47III  (5928)
1 site
A G C G C T T C G C G A
CpoI  (5655)
1 site
C G G W C C G G C C W G G C

Sticky ends from different CpoI sites may not be compatible.
CspI  (5655)
1 site
C G G W C C G G C C W G G C

Sticky ends from different CspI sites may not be compatible.
RsrII  (5655)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
Rsr2I  (5655)
1 site
C G G W C C G G C C W G G C

Sticky ends from different Rsr2I sites may not be compatible.
AspI  (5257)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by AspI may be hard to ligate.
Sticky ends from different AspI sites may not be compatible.
PflFI  (5257)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
PsyI  (5257)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PsyI may be hard to ligate.
Sticky ends from different PsyI sites may not be compatible.
Tth111I  (5257)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BalI  (5221)
1 site
T G G C C A A C C G G T
MlsI  (5221)
1 site
T G G C C A A C C G G T
MluNI  (5221)
1 site
T G G C C A A C C G G T
MscI  (5221)
1 site
T G G C C A A C C G G T
Msp20I  (5221)
1 site
T G G C C A A C C G G T
KflI  (1934)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
PpuMI  (1934)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
Psp5II  (1934)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different Psp5II sites may not be compatible.
PspPPI  (1934)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PspPPI sites may not be compatible.
SanDI  (1934)
1 site
G G G W C C C C C C W G G G

Sticky ends from different SanDI sites may not be compatible.
Bsp1407I  (2229)
1 site
T G T A C A A C A T G T
BsrGI  (2229)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BstAUI  (2229)
1 site
T G T A C A A C A T G T
SspBI  (2229)
1 site
T G T A C A A C